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3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells

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3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass : Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells. / Wang, Xiaoju; Molino, Binbin Zhang; Pitkänen, Sanna; Ojansivu, Miina; Xu, Chunlin; Hannula, Markus; Hyttinen, Jari; Miettinen, Susanna; Hupa, Leena; Wallace, Gordon.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 9, 18.07.2019, p. 4496-4510.

Research output: Contribution to journalArticleScientificpeer-review

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Wang, X, Molino, BZ, Pitkänen, S, Ojansivu, M, Xu, C, Hannula, M, Hyttinen, J, Miettinen, S, Hupa, L & Wallace, G 2019, '3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells', ACS Biomaterials Science and Engineering, vol. 5, no. 9, pp. 4496-4510. https://doi.org/10.1021/acsbiomaterials.9b00105

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Wang, Xiaoju ; Molino, Binbin Zhang ; Pitkänen, Sanna ; Ojansivu, Miina ; Xu, Chunlin ; Hannula, Markus ; Hyttinen, Jari ; Miettinen, Susanna ; Hupa, Leena ; Wallace, Gordon. / 3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass : Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 9. pp. 4496-4510.

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@article{19c994272b7c456fb863f99d3aca115e,
title = "3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells",
abstract = "The local delivery of Cu2+ from copper-doped bioactive glass (Cu-BaG) was combined with 3D printing of polycaprolactone (PCL) scaffolds for its potent angiogenic effect in bone tissue engineering. PCL and Cu-BaG were, respectively, dissolved and dispersed in acetone to formulate a moderately homogeneous ink. The PCL/Cu-BaG scaffolds were fabricated via direct ink writing into a cold ethanol bath. The architecture of the printed scaffolds, including strut diameter, strut spacing, and porosity, were investigated and characterized. The PCL/Cu-BaG scaffolds showed a Cu-BaG content-dependent mechanical property, as the compressive Young's modulus ranged from 7 to 13 MPa at an apparent porosity of 60{\%}. The ion dissolution behavior in simulated body fluid was evaluated, and the hydroxyapatite-like precipitation on the strut surface was confirmed. Furthermore, the cytocompatibility of the PCL/Cu-BaG scaffolds was assessed in human bone marrow stem cell (hBMSC) culture, and a dose-dependent cytotoxicity of Cu2+ was observed. Here, the PCL/BaG scaffold induced the higher expression of late osteogenic genes OSTEOCALCIN and DLX5 in comparison to the PCL scaffold. The doping of Cu2+ in BaG elicited higher expression of the early osteogenic marker gene RUNX2a but decreased the expression of late osteogenic marker genes OSTEOCALCIN and DLX5 in comparison to the PCL/BaG scaffold, demonstrating the suppressing effect of Cu2+ on osteogenic differentiation of hBMSCs. In a coculture of hBMSCs and human umbilical vein endothelial cells, both the PCL/BaG and PCL/Cu-BaG scaffolds stimulated the formation of a denser tubule network, compared to the PCL scaffold. Meanwhile, only slightly higher gene expression of vWF was observed with the PCL/Cu-BaG scaffold than with the PCL/BaG scaffold, indicating the potent angiogenic effect of the released Cu2+.",
keywords = "3D printing, angiogenesis, coculture of mesenchymal stem cells and endothelial cells, copper-doped bioactive glass, gradient porosity, polycaprolactone, tissue engineering scaffold",
author = "Xiaoju Wang and Molino, {Binbin Zhang} and Sanna Pitk{\"a}nen and Miina Ojansivu and Chunlin Xu and Markus Hannula and Jari Hyttinen and Susanna Miettinen and Leena Hupa and Gordon Wallace",
year = "2019",
month = "7",
day = "18",
doi = "10.1021/acsbiomaterials.9b00105",
language = "English",
volume = "5",
pages = "4496--4510",
journal = "ACS Biomaterials Science & Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "9",

}

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TY - JOUR

T1 - 3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass

T2 - Architecture Engineering with Additive Manufacturing and Cellular Assessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells

AU - Wang, Xiaoju

AU - Molino, Binbin Zhang

AU - Pitkänen, Sanna

AU - Ojansivu, Miina

AU - Xu, Chunlin

AU - Hannula, Markus

AU - Hyttinen, Jari

AU - Miettinen, Susanna

AU - Hupa, Leena

AU - Wallace, Gordon

PY - 2019/7/18

Y1 - 2019/7/18

N2 - The local delivery of Cu2+ from copper-doped bioactive glass (Cu-BaG) was combined with 3D printing of polycaprolactone (PCL) scaffolds for its potent angiogenic effect in bone tissue engineering. PCL and Cu-BaG were, respectively, dissolved and dispersed in acetone to formulate a moderately homogeneous ink. The PCL/Cu-BaG scaffolds were fabricated via direct ink writing into a cold ethanol bath. The architecture of the printed scaffolds, including strut diameter, strut spacing, and porosity, were investigated and characterized. The PCL/Cu-BaG scaffolds showed a Cu-BaG content-dependent mechanical property, as the compressive Young's modulus ranged from 7 to 13 MPa at an apparent porosity of 60%. The ion dissolution behavior in simulated body fluid was evaluated, and the hydroxyapatite-like precipitation on the strut surface was confirmed. Furthermore, the cytocompatibility of the PCL/Cu-BaG scaffolds was assessed in human bone marrow stem cell (hBMSC) culture, and a dose-dependent cytotoxicity of Cu2+ was observed. Here, the PCL/BaG scaffold induced the higher expression of late osteogenic genes OSTEOCALCIN and DLX5 in comparison to the PCL scaffold. The doping of Cu2+ in BaG elicited higher expression of the early osteogenic marker gene RUNX2a but decreased the expression of late osteogenic marker genes OSTEOCALCIN and DLX5 in comparison to the PCL/BaG scaffold, demonstrating the suppressing effect of Cu2+ on osteogenic differentiation of hBMSCs. In a coculture of hBMSCs and human umbilical vein endothelial cells, both the PCL/BaG and PCL/Cu-BaG scaffolds stimulated the formation of a denser tubule network, compared to the PCL scaffold. Meanwhile, only slightly higher gene expression of vWF was observed with the PCL/Cu-BaG scaffold than with the PCL/BaG scaffold, indicating the potent angiogenic effect of the released Cu2+.

AB - The local delivery of Cu2+ from copper-doped bioactive glass (Cu-BaG) was combined with 3D printing of polycaprolactone (PCL) scaffolds for its potent angiogenic effect in bone tissue engineering. PCL and Cu-BaG were, respectively, dissolved and dispersed in acetone to formulate a moderately homogeneous ink. The PCL/Cu-BaG scaffolds were fabricated via direct ink writing into a cold ethanol bath. The architecture of the printed scaffolds, including strut diameter, strut spacing, and porosity, were investigated and characterized. The PCL/Cu-BaG scaffolds showed a Cu-BaG content-dependent mechanical property, as the compressive Young's modulus ranged from 7 to 13 MPa at an apparent porosity of 60%. The ion dissolution behavior in simulated body fluid was evaluated, and the hydroxyapatite-like precipitation on the strut surface was confirmed. Furthermore, the cytocompatibility of the PCL/Cu-BaG scaffolds was assessed in human bone marrow stem cell (hBMSC) culture, and a dose-dependent cytotoxicity of Cu2+ was observed. Here, the PCL/BaG scaffold induced the higher expression of late osteogenic genes OSTEOCALCIN and DLX5 in comparison to the PCL scaffold. The doping of Cu2+ in BaG elicited higher expression of the early osteogenic marker gene RUNX2a but decreased the expression of late osteogenic marker genes OSTEOCALCIN and DLX5 in comparison to the PCL/BaG scaffold, demonstrating the suppressing effect of Cu2+ on osteogenic differentiation of hBMSCs. In a coculture of hBMSCs and human umbilical vein endothelial cells, both the PCL/BaG and PCL/Cu-BaG scaffolds stimulated the formation of a denser tubule network, compared to the PCL scaffold. Meanwhile, only slightly higher gene expression of vWF was observed with the PCL/Cu-BaG scaffold than with the PCL/BaG scaffold, indicating the potent angiogenic effect of the released Cu2+.

KW - 3D printing

KW - angiogenesis

KW - coculture of mesenchymal stem cells and endothelial cells

KW - copper-doped bioactive glass

KW - gradient porosity

KW - polycaprolactone

KW - tissue engineering scaffold

U2 - 10.1021/acsbiomaterials.9b00105

DO - 10.1021/acsbiomaterials.9b00105

M3 - Article

VL - 5

SP - 4496

EP - 4510

JO - ACS Biomaterials Science & Engineering

JF - ACS Biomaterials Science & Engineering

SN - 2373-9878

IS - 9

ER -