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A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons

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A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons. / Zhang, Dawei; Pekkanen-Mattila, Mari; Shahsavani, Mansoureh; Falk, Anna; Teixeira, Ana I.; Herland, Anna.

In: Biomaterials, Vol. 35, No. 5, 02.2014, p. 1420-1428.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Zhang, D, Pekkanen-Mattila, M, Shahsavani, M, Falk, A, Teixeira, AI & Herland, A 2014, 'A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons', Biomaterials, vol. 35, no. 5, pp. 1420-1428. https://doi.org/10.1016/j.biomaterials.2013.11.028

APA

Zhang, D., Pekkanen-Mattila, M., Shahsavani, M., Falk, A., Teixeira, A. I., & Herland, A. (2014). A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons. Biomaterials, 35(5), 1420-1428. https://doi.org/10.1016/j.biomaterials.2013.11.028

Vancouver

Zhang D, Pekkanen-Mattila M, Shahsavani M, Falk A, Teixeira AI, Herland A. A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons. Biomaterials. 2014 Feb;35(5):1420-1428. https://doi.org/10.1016/j.biomaterials.2013.11.028

Author

Zhang, Dawei ; Pekkanen-Mattila, Mari ; Shahsavani, Mansoureh ; Falk, Anna ; Teixeira, Ana I. ; Herland, Anna. / A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons. In: Biomaterials. 2014 ; Vol. 35, No. 5. pp. 1420-1428.

Bibtex - Download

@article{4a4ce6a53e4d4248a152fc27ccb80c02,
title = "A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons",
abstract = "The recent progress in stem cell techniques has broadened the horizon for invitro disease modeling. For desired invivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce invivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed clear effects on F-actin associated proteins, connected to the disease processes. We propose that the 3D invitro model has higher resemblance to the AD pathology than conventional 2D cultures and could be used in further studies of the disease.",
keywords = "3D culture, Alzheimer's disease, IPSCs, Mechanotransduction, Neuron, Self-assembling peptide",
author = "Dawei Zhang and Mari Pekkanen-Mattila and Mansoureh Shahsavani and Anna Falk and Teixeira, {Ana I.} and Anna Herland",
year = "2014",
month = "2",
doi = "10.1016/j.biomaterials.2013.11.028",
language = "English",
volume = "35",
pages = "1420--1428",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons

AU - Zhang, Dawei

AU - Pekkanen-Mattila, Mari

AU - Shahsavani, Mansoureh

AU - Falk, Anna

AU - Teixeira, Ana I.

AU - Herland, Anna

PY - 2014/2

Y1 - 2014/2

N2 - The recent progress in stem cell techniques has broadened the horizon for invitro disease modeling. For desired invivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce invivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed clear effects on F-actin associated proteins, connected to the disease processes. We propose that the 3D invitro model has higher resemblance to the AD pathology than conventional 2D cultures and could be used in further studies of the disease.

AB - The recent progress in stem cell techniques has broadened the horizon for invitro disease modeling. For desired invivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce invivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed clear effects on F-actin associated proteins, connected to the disease processes. We propose that the 3D invitro model has higher resemblance to the AD pathology than conventional 2D cultures and could be used in further studies of the disease.

KW - 3D culture

KW - Alzheimer's disease

KW - IPSCs

KW - Mechanotransduction

KW - Neuron

KW - Self-assembling peptide

UR - http://www.scopus.com/inward/record.url?scp=84890173885&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.11.028

DO - 10.1016/j.biomaterials.2013.11.028

M3 - Article

VL - 35

SP - 1420

EP - 1428

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 5

ER -