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A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances

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A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances. / Tienaho, Jenni; Poikulainen, Emmi; Sarjala, Tytti; Muilu-Mäkelä, Riina; Santala, Ville; Karp, Matti.

In: Photochemistry and Photobiology, Vol. 94, No. 6, 2018, p. 1273-1280.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Tienaho, J, Poikulainen, E, Sarjala, T, Muilu-Mäkelä, R, Santala, V & Karp, M 2018, 'A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances', Photochemistry and Photobiology, vol. 94, no. 6, pp. 1273-1280. https://doi.org/10.1111/php.12954

APA

Tienaho, J., Poikulainen, E., Sarjala, T., Muilu-Mäkelä, R., Santala, V., & Karp, M. (2018). A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances. Photochemistry and Photobiology, 94(6), 1273-1280. https://doi.org/10.1111/php.12954

Vancouver

Tienaho J, Poikulainen E, Sarjala T, Muilu-Mäkelä R, Santala V, Karp M. A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances. Photochemistry and Photobiology. 2018;94(6):1273-1280. https://doi.org/10.1111/php.12954

Author

Tienaho, Jenni ; Poikulainen, Emmi ; Sarjala, Tytti ; Muilu-Mäkelä, Riina ; Santala, Ville ; Karp, Matti. / A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances. In: Photochemistry and Photobiology. 2018 ; Vol. 94, No. 6. pp. 1273-1280.

Bibtex - Download

@article{b79887a9003f4ec1a2a3b0e272f12840,
title = "A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances",
abstract = "Ultraviolet radiation (UV-R) causes genotoxic and aging effects on skin, and sunscreens are used to alleviate the damage. However, sunscreens contain synthetic shielding agents that can cause harmful effects in the environment. Nature-derived substances may have potential as replacement materials for the harmful sunscreen chemicals. However, screening of a broad range of samples is tedious, and often requires a separate genotoxicity assessment. We describe a simple microplate technique for the screening of UV protective substances using a recombinant Escherichia coli biosensor. Both absorbance-based and bioactivity-based shields can be detected with simultaneous information about the sample genotoxicity. With this technique, a controversial sunscreen compound, oxybenzone offers physical or absorbance-based shield but appears genotoxic at higher concentrations (3.3 mg/mL). We also demonstrate that pine needle extract (PiNe) shields the biosensor from UV-R in a dose-dependent manner without showing genotoxicity. The physical shield of 5 mg/mL PiNe was similar to that of one of the most common UV-shielding compound TiO2 concentration 0.80 mg/mL. The bioactivity-based shield of PiNe also reaches the extent of the physical shield with the highest concentration (3.3 mg/mL). We conclude that our technique is suitable in detecting the UV-shielding potential of natural substances, and gives simultaneous information on genotoxicity.",
author = "Jenni Tienaho and Emmi Poikulainen and Tytti Sarjala and Riina Muilu-M{\"a}kel{\"a} and Ville Santala and Matti Karp",
note = "INT=keb,{"}Poikulainen, Emmi{"}",
year = "2018",
doi = "10.1111/php.12954",
language = "English",
volume = "94",
pages = "1273--1280",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A Bioscreening Technique for Ultraviolet Irradiation Protective Natural Substances

AU - Tienaho, Jenni

AU - Poikulainen, Emmi

AU - Sarjala, Tytti

AU - Muilu-Mäkelä, Riina

AU - Santala, Ville

AU - Karp, Matti

N1 - INT=keb,"Poikulainen, Emmi"

PY - 2018

Y1 - 2018

N2 - Ultraviolet radiation (UV-R) causes genotoxic and aging effects on skin, and sunscreens are used to alleviate the damage. However, sunscreens contain synthetic shielding agents that can cause harmful effects in the environment. Nature-derived substances may have potential as replacement materials for the harmful sunscreen chemicals. However, screening of a broad range of samples is tedious, and often requires a separate genotoxicity assessment. We describe a simple microplate technique for the screening of UV protective substances using a recombinant Escherichia coli biosensor. Both absorbance-based and bioactivity-based shields can be detected with simultaneous information about the sample genotoxicity. With this technique, a controversial sunscreen compound, oxybenzone offers physical or absorbance-based shield but appears genotoxic at higher concentrations (3.3 mg/mL). We also demonstrate that pine needle extract (PiNe) shields the biosensor from UV-R in a dose-dependent manner without showing genotoxicity. The physical shield of 5 mg/mL PiNe was similar to that of one of the most common UV-shielding compound TiO2 concentration 0.80 mg/mL. The bioactivity-based shield of PiNe also reaches the extent of the physical shield with the highest concentration (3.3 mg/mL). We conclude that our technique is suitable in detecting the UV-shielding potential of natural substances, and gives simultaneous information on genotoxicity.

AB - Ultraviolet radiation (UV-R) causes genotoxic and aging effects on skin, and sunscreens are used to alleviate the damage. However, sunscreens contain synthetic shielding agents that can cause harmful effects in the environment. Nature-derived substances may have potential as replacement materials for the harmful sunscreen chemicals. However, screening of a broad range of samples is tedious, and often requires a separate genotoxicity assessment. We describe a simple microplate technique for the screening of UV protective substances using a recombinant Escherichia coli biosensor. Both absorbance-based and bioactivity-based shields can be detected with simultaneous information about the sample genotoxicity. With this technique, a controversial sunscreen compound, oxybenzone offers physical or absorbance-based shield but appears genotoxic at higher concentrations (3.3 mg/mL). We also demonstrate that pine needle extract (PiNe) shields the biosensor from UV-R in a dose-dependent manner without showing genotoxicity. The physical shield of 5 mg/mL PiNe was similar to that of one of the most common UV-shielding compound TiO2 concentration 0.80 mg/mL. The bioactivity-based shield of PiNe also reaches the extent of the physical shield with the highest concentration (3.3 mg/mL). We conclude that our technique is suitable in detecting the UV-shielding potential of natural substances, and gives simultaneous information on genotoxicity.

U2 - 10.1111/php.12954

DO - 10.1111/php.12954

M3 - Article

VL - 94

SP - 1273

EP - 1280

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 6

ER -