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A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing

Research output: Contribution to journalArticleScientificpeer-review

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A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing. / Ristola, Mervi; Sukki, Lassi; Azevedo, Maria Manuela; Seixas, Ana Isabel; Relvas, João Bettencourt; Narkilahti, Susanna; Kallio, Pasi.

In: Journal of Micromechanics and Microengineering, Vol. 6, No. 29, 03.05.2019, p. 1-13.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Ristola, M, Sukki, L, Azevedo, MM, Seixas, AI, Relvas, JB, Narkilahti, S & Kallio, P 2019, 'A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing' Journal of Micromechanics and Microengineering, vol. 6, no. 29, pp. 1-13. https://doi.org/10.1088/1361-6439/ab16a7

APA

Ristola, M., Sukki, L., Azevedo, M. M., Seixas, A. I., Relvas, JB., Narkilahti, S., & Kallio, P. (2019). A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing. Journal of Micromechanics and Microengineering, 6(29), 1-13. https://doi.org/10.1088/1361-6439/ab16a7

Vancouver

Ristola M, Sukki L, Azevedo MM, Seixas AI, Relvas JB, Narkilahti S et al. A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing. Journal of Micromechanics and Microengineering. 2019 May 3;6(29):1-13. https://doi.org/10.1088/1361-6439/ab16a7

Author

Ristola, Mervi ; Sukki, Lassi ; Azevedo, Maria Manuela ; Seixas, Ana Isabel ; Relvas, João Bettencourt ; Narkilahti, Susanna ; Kallio, Pasi. / A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing. In: Journal of Micromechanics and Microengineering. 2019 ; Vol. 6, No. 29. pp. 1-13.

Bibtex - Download

@article{8a3c741e8b614809ad4a0788a38512e3,
title = "A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing",
abstract = "Microfluidics devices for co-culturing neurons and oligodendrocytes represent an important in vitro research tool to decipher myelination mechanisms in health and disease and in the identification of novel treatments for myelin diseases. In reported devices using primary rodent cells, the spontaneous formation of myelin sheaths has been challenging and random orientation of neurites impede the analysis of myelination. Furthermore, fabrication methods for devices show limitations, highlighting the need for novel in vitro cell-based myelination models. In the present study, we describe a compartmentalized cell culture device targeted for neuron-oligodendrocyte co-culturing and myelination studies. In the device, neurites from primary rat dorsal root ganglion (DRG) neurons were capable of forming aligned dense networks in a specific compartment that was physically isolated from neuronal somas. Co-culture of rat DRG neurons and oligodendrocytes, a well-known model to study myelination in vitro, led to interactions between oligodendrocytes and neurites in the device, and the deposition of myelin segments in an aligned distribution was spontaneously formed. For the fabrication of the device, we present a new method that produces polydimethylsiloxane (PDMS)—based devices possessing an open compartment design. The proposed fabrication method takes advantage of an SU-8 photolithography process and 3D printing for mould fabrication. Both the microscale and macroscale features are replicated from the same mould, allowing devices to be produced with high precision and repeatability. The proposed device is applicable for long-term cell culturing, live-cell imaging, and by enhancing aligned myelin distribution, it is a promising tool for experimental setups that address diverse biological questions in the field of myelin research.",
keywords = "microfluidics, microfabrication, 3D printing, Co-culture, myelination",
author = "Mervi Ristola and Lassi Sukki and Azevedo, {Maria Manuela} and Seixas, {Ana Isabel} and Jo{\~a}o Bettencourt Relvas and Susanna Narkilahti and Pasi Kallio",
note = "dupl=47545691",
year = "2019",
month = "5",
day = "3",
doi = "10.1088/1361-6439/ab16a7",
language = "English",
volume = "6",
pages = "1--13",
journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",
publisher = "IOP Publishing",
number = "29",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing

AU - Ristola, Mervi

AU - Sukki, Lassi

AU - Azevedo, Maria Manuela

AU - Seixas, Ana Isabel

AU - Relvas, João Bettencourt

AU - Narkilahti, Susanna

AU - Kallio, Pasi

N1 - dupl=47545691

PY - 2019/5/3

Y1 - 2019/5/3

N2 - Microfluidics devices for co-culturing neurons and oligodendrocytes represent an important in vitro research tool to decipher myelination mechanisms in health and disease and in the identification of novel treatments for myelin diseases. In reported devices using primary rodent cells, the spontaneous formation of myelin sheaths has been challenging and random orientation of neurites impede the analysis of myelination. Furthermore, fabrication methods for devices show limitations, highlighting the need for novel in vitro cell-based myelination models. In the present study, we describe a compartmentalized cell culture device targeted for neuron-oligodendrocyte co-culturing and myelination studies. In the device, neurites from primary rat dorsal root ganglion (DRG) neurons were capable of forming aligned dense networks in a specific compartment that was physically isolated from neuronal somas. Co-culture of rat DRG neurons and oligodendrocytes, a well-known model to study myelination in vitro, led to interactions between oligodendrocytes and neurites in the device, and the deposition of myelin segments in an aligned distribution was spontaneously formed. For the fabrication of the device, we present a new method that produces polydimethylsiloxane (PDMS)—based devices possessing an open compartment design. The proposed fabrication method takes advantage of an SU-8 photolithography process and 3D printing for mould fabrication. Both the microscale and macroscale features are replicated from the same mould, allowing devices to be produced with high precision and repeatability. The proposed device is applicable for long-term cell culturing, live-cell imaging, and by enhancing aligned myelin distribution, it is a promising tool for experimental setups that address diverse biological questions in the field of myelin research.

AB - Microfluidics devices for co-culturing neurons and oligodendrocytes represent an important in vitro research tool to decipher myelination mechanisms in health and disease and in the identification of novel treatments for myelin diseases. In reported devices using primary rodent cells, the spontaneous formation of myelin sheaths has been challenging and random orientation of neurites impede the analysis of myelination. Furthermore, fabrication methods for devices show limitations, highlighting the need for novel in vitro cell-based myelination models. In the present study, we describe a compartmentalized cell culture device targeted for neuron-oligodendrocyte co-culturing and myelination studies. In the device, neurites from primary rat dorsal root ganglion (DRG) neurons were capable of forming aligned dense networks in a specific compartment that was physically isolated from neuronal somas. Co-culture of rat DRG neurons and oligodendrocytes, a well-known model to study myelination in vitro, led to interactions between oligodendrocytes and neurites in the device, and the deposition of myelin segments in an aligned distribution was spontaneously formed. For the fabrication of the device, we present a new method that produces polydimethylsiloxane (PDMS)—based devices possessing an open compartment design. The proposed fabrication method takes advantage of an SU-8 photolithography process and 3D printing for mould fabrication. Both the microscale and macroscale features are replicated from the same mould, allowing devices to be produced with high precision and repeatability. The proposed device is applicable for long-term cell culturing, live-cell imaging, and by enhancing aligned myelin distribution, it is a promising tool for experimental setups that address diverse biological questions in the field of myelin research.

KW - microfluidics

KW - microfabrication

KW - 3D printing

KW - Co-culture

KW - myelination

U2 - 10.1088/1361-6439/ab16a7

DO - 10.1088/1361-6439/ab16a7

M3 - Article

VL - 6

SP - 1

EP - 13

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 29

ER -