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Advances in Human Stem Cell-Derived Neuronal Cell Culturing and Analysis

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Details

Original languageEnglish
Title of host publicationIn Vitro Neuronal Networks
Subtitle of host publicationFrom Culturing Methods to Neuro-Technological Applications
PublisherSpringer New York LLC
Pages299-329
Number of pages31
ISBN (Electronic)978-3-030-11135-9
ISBN (Print)978-3-030-11134-2
DOIs
Publication statusPublished - 2019
Publication typeA3 Part of a book or another research book

Publication series

NameAdvances in Neurobiology
Volume22
ISSN (Print)2190-5215

Abstract

This chapter provides an overview of the current stage of human in vitro functional neuronal cultures, their biological application areas, and modalities to analyze their behavior. During the last 10 years, this research area has changed from being practically non-existent to one that is facing high expectations. Here, we present a case study as a comprehensive short history of this process based on extensive studies conducted at NeuroGroup (University of Tampere) and Computational Biophysics and Imaging Group (Tampere University of Technology), ranging from the differentiation and culturing of human pluripotent stem cell (hPSC)-derived neuronal networks to their electrophysiological analysis. After an introduction to neuronal differentiation in hPSCs, we review our work on their functionality and approaches for extending cultures from 2D to 3D systems. Thereafter, we discuss our target applications in neuronal developmental modeling, toxicology, drug screening, and disease modeling. The development of signal analysis methods was required due to the unique functional and developmental properties of hPSC-derived neuronal cells and networks, which separate them from their much-used rodent counterparts. Accordingly, a line of microelectrode array (MEA) signal analysis methods was developed. This work included the development of action potential spike detection methods, entropy-based methods and additional methods for burst detection and quantification, joint analysis of spikes and bursts to analyze the spike waveform compositions of bursts, assessment methods for network synchronization, and computational simulations of synapses and neuronal networks.

Keywords

  • Human neurons, Human pluripotent stem cells, Microelectrode arrays, Signal analysis

Publication forum classification

Field of science, Statistics Finland