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Alkane and wax ester production from lignin-related aromatic compounds

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Alkane and wax ester production from lignin-related aromatic compounds. / Salmela, Milla; Lehtinen, Tapio; Efimova, Elena; Santala, Suvi; Santala, Ville.

In: Biotechnology and Bioengineering, Vol. 116, No. 8, 01.08.2019, p. 1934-1945.

Research output: Contribution to journalArticleScientificpeer-review

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Salmela, M, Lehtinen, T, Efimova, E, Santala, S & Santala, V 2019, 'Alkane and wax ester production from lignin-related aromatic compounds', Biotechnology and Bioengineering, vol. 116, no. 8, pp. 1934-1945. https://doi.org/10.1002/bit.27005

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Vancouver

Salmela M, Lehtinen T, Efimova E, Santala S, Santala V. Alkane and wax ester production from lignin-related aromatic compounds. Biotechnology and Bioengineering. 2019 Aug 1;116(8):1934-1945. https://doi.org/10.1002/bit.27005

Author

Salmela, Milla ; Lehtinen, Tapio ; Efimova, Elena ; Santala, Suvi ; Santala, Ville. / Alkane and wax ester production from lignin-related aromatic compounds. In: Biotechnology and Bioengineering. 2019 ; Vol. 116, No. 8. pp. 1934-1945.

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@article{9722bec3937746b982cc2be150717b76,
title = "Alkane and wax ester production from lignin-related aromatic compounds",
abstract = "Lignin has potential as a sustainable feedstock for microbial production of industrially relevant molecules. However, the required lignin depolymerization yields a heterogenic mixture of aromatic monomers that are challenging substrates for the microorganisms commonly used in the industry. Here, we investigated the properties of lignin-related aromatic compounds (LRAs), namely coumarate, ferulate, and caffeate, in the synthesis of biomass and products in an LRA-utilizing bacterial host Acinetobacter baylyi ADP1. The biosynthesis products, wax esters, and alkanes are relevant compounds for the chemical and fuel industries. Here, wax esters were produced by a native pathway of ADP1, whereas alkanes were produced by a synthetic pathway introduced to the host. Using individual LRAs as substrates, the growth and product formation were monitored with internal biosensors and off-line analytics. Of the tested LRAs, coumarate was the most propitious in terms of product synthesis. Wax esters were produced from coumarate with yield and titer of 37 mg/gcoumarate and 202 mg/L, whereas alkanes were produced with a yield of 62.3 µg /gcoumarate and titer of 152 µg/L. This study demonstrates the microbial preference for certain LRAs and highlights the potential of A. baylyi ADP1 as a host for LRA upgrading to value-added products.",
keywords = "Acinetobacter baylyi ADP1, alkane, lignin, wax ester",
author = "Milla Salmela and Tapio Lehtinen and Elena Efimova and Suvi Santala and Ville Santala",
year = "2019",
month = "8",
day = "1",
doi = "10.1002/bit.27005",
language = "English",
volume = "116",
pages = "1934--1945",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley",
number = "8",

}

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TY - JOUR

T1 - Alkane and wax ester production from lignin-related aromatic compounds

AU - Salmela, Milla

AU - Lehtinen, Tapio

AU - Efimova, Elena

AU - Santala, Suvi

AU - Santala, Ville

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Lignin has potential as a sustainable feedstock for microbial production of industrially relevant molecules. However, the required lignin depolymerization yields a heterogenic mixture of aromatic monomers that are challenging substrates for the microorganisms commonly used in the industry. Here, we investigated the properties of lignin-related aromatic compounds (LRAs), namely coumarate, ferulate, and caffeate, in the synthesis of biomass and products in an LRA-utilizing bacterial host Acinetobacter baylyi ADP1. The biosynthesis products, wax esters, and alkanes are relevant compounds for the chemical and fuel industries. Here, wax esters were produced by a native pathway of ADP1, whereas alkanes were produced by a synthetic pathway introduced to the host. Using individual LRAs as substrates, the growth and product formation were monitored with internal biosensors and off-line analytics. Of the tested LRAs, coumarate was the most propitious in terms of product synthesis. Wax esters were produced from coumarate with yield and titer of 37 mg/gcoumarate and 202 mg/L, whereas alkanes were produced with a yield of 62.3 µg /gcoumarate and titer of 152 µg/L. This study demonstrates the microbial preference for certain LRAs and highlights the potential of A. baylyi ADP1 as a host for LRA upgrading to value-added products.

AB - Lignin has potential as a sustainable feedstock for microbial production of industrially relevant molecules. However, the required lignin depolymerization yields a heterogenic mixture of aromatic monomers that are challenging substrates for the microorganisms commonly used in the industry. Here, we investigated the properties of lignin-related aromatic compounds (LRAs), namely coumarate, ferulate, and caffeate, in the synthesis of biomass and products in an LRA-utilizing bacterial host Acinetobacter baylyi ADP1. The biosynthesis products, wax esters, and alkanes are relevant compounds for the chemical and fuel industries. Here, wax esters were produced by a native pathway of ADP1, whereas alkanes were produced by a synthetic pathway introduced to the host. Using individual LRAs as substrates, the growth and product formation were monitored with internal biosensors and off-line analytics. Of the tested LRAs, coumarate was the most propitious in terms of product synthesis. Wax esters were produced from coumarate with yield and titer of 37 mg/gcoumarate and 202 mg/L, whereas alkanes were produced with a yield of 62.3 µg /gcoumarate and titer of 152 µg/L. This study demonstrates the microbial preference for certain LRAs and highlights the potential of A. baylyi ADP1 as a host for LRA upgrading to value-added products.

KW - Acinetobacter baylyi ADP1

KW - alkane

KW - lignin

KW - wax ester

U2 - 10.1002/bit.27005

DO - 10.1002/bit.27005

M3 - Article

VL - 116

SP - 1934

EP - 1945

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 8

ER -