An efficient auxin-inducible degron system with low basal degradation in human cells
Research output: Contribution to journal › Article › Scientific › peer-review
Details
| Original language | English |
|---|---|
| Pages (from-to) | 866-869 |
| Number of pages | 4 |
| Journal | Nature Methods |
| Volume | 16 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1 Sep 2019 |
| Publication type | A1 Journal article-refereed |
Abstract
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.