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An efficient auxin-inducible degron system with low basal degradation in human cells

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Original languageEnglish
Pages (from-to)866-869
Number of pages4
JournalNature Methods
Issue number9
Publication statusPublished - 1 Sep 2019
Publication typeA1 Journal article-refereed


Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.

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Field of science, Statistics Finland