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Anaerobic treatment of LCFA-containing synthetic dairy wastewater at 20 °C: Process performance and microbial community dynamics

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Anaerobic treatment of LCFA-containing synthetic dairy wastewater at 20 °C : Process performance and microbial community dynamics. / Singh, Suniti; Rinta-Kanto, Johanna M.; Kettunen, Riitta; Tolvanen, Henrik; Lens, Piet; Collins, Gavin; Kokko, Marika; Rintala, Jukka.

In: Science of the Total Environment, Vol. 691, 15.11.2019, p. 960-968.

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@article{b637da371d4b45febf6dbc5fa9cab624,
title = "Anaerobic treatment of LCFA-containing synthetic dairy wastewater at 20 °C: Process performance and microbial community dynamics",
abstract = "Facilitating anaerobic degradation of long-chain fatty acids (LCFA) is key for tapping the high methane production potential of the fats, oil and grease (FOG) content of dairy wastewaters. In this study, the feasibility of using high-rate granular sludge reactors for the treatment of mixed LCFA-containing synthetic dairy wastewater (SDW) was assessed at 20 °C. The effects of the LCFA concentration (33–45{\%} of COD) and organic loading rates (2–3 gCOD/L·d) were determined using three parallel expanded granular sludge bed reactors. For the first time, long term anaerobic treatment of LCFA-containing feed at 20 °C was shown to be feasible and was linked to the microbial community dynamics in high-rate reactors. During a two-month operation, a soluble COD removal of 84–91{\%} and COD to methane conversion of 44–51{\%} was obtained. However, granular sludge flotation and washout occurred after two months in all reactors without volatile fatty acids (VFA) accumulation, emphasizing the need for sludge retention for long-term granular sludge reactor operation with LCFA-containing feed at low ambient temperatures. The temporal shifts in microbial community structure were studied in the high-rate treatment of SDW, and the process disturbances (elevated LCFA loading, LCFA accumulation, and batch operation) were found to decrease the microbial community diversity. The relative abundance of Methanosaeta increased with higher LCFA accumulation in the settled and flotation layer granules in the three reactors, therefore, acetoclastic methanogenesis was found to be crucial for the high-rate treatment of SDW at 20 °C. This study provides an initial understanding of the continuous anaerobic treatment of LCFA-containing industrial wastewaters at low ambient temperatures.",
keywords = "Dairy wastewater, Expanded granular sludge bed (EGSB), Granule disintegration, Long chain fatty acids (LCFA), Methanogenesis pathway, Microbial community dynamics",
author = "Suniti Singh and Rinta-Kanto, {Johanna M.} and Riitta Kettunen and Henrik Tolvanen and Piet Lens and Gavin Collins and Marika Kokko and Jukka Rintala",
year = "2019",
month = "11",
day = "15",
doi = "10.1016/j.scitotenv.2019.07.136",
language = "English",
volume = "691",
pages = "960--968",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier Science B.V.",

}

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TY - JOUR

T1 - Anaerobic treatment of LCFA-containing synthetic dairy wastewater at 20 °C

T2 - Process performance and microbial community dynamics

AU - Singh, Suniti

AU - Rinta-Kanto, Johanna M.

AU - Kettunen, Riitta

AU - Tolvanen, Henrik

AU - Lens, Piet

AU - Collins, Gavin

AU - Kokko, Marika

AU - Rintala, Jukka

PY - 2019/11/15

Y1 - 2019/11/15

N2 - Facilitating anaerobic degradation of long-chain fatty acids (LCFA) is key for tapping the high methane production potential of the fats, oil and grease (FOG) content of dairy wastewaters. In this study, the feasibility of using high-rate granular sludge reactors for the treatment of mixed LCFA-containing synthetic dairy wastewater (SDW) was assessed at 20 °C. The effects of the LCFA concentration (33–45% of COD) and organic loading rates (2–3 gCOD/L·d) were determined using three parallel expanded granular sludge bed reactors. For the first time, long term anaerobic treatment of LCFA-containing feed at 20 °C was shown to be feasible and was linked to the microbial community dynamics in high-rate reactors. During a two-month operation, a soluble COD removal of 84–91% and COD to methane conversion of 44–51% was obtained. However, granular sludge flotation and washout occurred after two months in all reactors without volatile fatty acids (VFA) accumulation, emphasizing the need for sludge retention for long-term granular sludge reactor operation with LCFA-containing feed at low ambient temperatures. The temporal shifts in microbial community structure were studied in the high-rate treatment of SDW, and the process disturbances (elevated LCFA loading, LCFA accumulation, and batch operation) were found to decrease the microbial community diversity. The relative abundance of Methanosaeta increased with higher LCFA accumulation in the settled and flotation layer granules in the three reactors, therefore, acetoclastic methanogenesis was found to be crucial for the high-rate treatment of SDW at 20 °C. This study provides an initial understanding of the continuous anaerobic treatment of LCFA-containing industrial wastewaters at low ambient temperatures.

AB - Facilitating anaerobic degradation of long-chain fatty acids (LCFA) is key for tapping the high methane production potential of the fats, oil and grease (FOG) content of dairy wastewaters. In this study, the feasibility of using high-rate granular sludge reactors for the treatment of mixed LCFA-containing synthetic dairy wastewater (SDW) was assessed at 20 °C. The effects of the LCFA concentration (33–45% of COD) and organic loading rates (2–3 gCOD/L·d) were determined using three parallel expanded granular sludge bed reactors. For the first time, long term anaerobic treatment of LCFA-containing feed at 20 °C was shown to be feasible and was linked to the microbial community dynamics in high-rate reactors. During a two-month operation, a soluble COD removal of 84–91% and COD to methane conversion of 44–51% was obtained. However, granular sludge flotation and washout occurred after two months in all reactors without volatile fatty acids (VFA) accumulation, emphasizing the need for sludge retention for long-term granular sludge reactor operation with LCFA-containing feed at low ambient temperatures. The temporal shifts in microbial community structure were studied in the high-rate treatment of SDW, and the process disturbances (elevated LCFA loading, LCFA accumulation, and batch operation) were found to decrease the microbial community diversity. The relative abundance of Methanosaeta increased with higher LCFA accumulation in the settled and flotation layer granules in the three reactors, therefore, acetoclastic methanogenesis was found to be crucial for the high-rate treatment of SDW at 20 °C. This study provides an initial understanding of the continuous anaerobic treatment of LCFA-containing industrial wastewaters at low ambient temperatures.

KW - Dairy wastewater

KW - Expanded granular sludge bed (EGSB)

KW - Granule disintegration

KW - Long chain fatty acids (LCFA)

KW - Methanogenesis pathway

KW - Microbial community dynamics

U2 - 10.1016/j.scitotenv.2019.07.136

DO - 10.1016/j.scitotenv.2019.07.136

M3 - Article

VL - 691

SP - 960

EP - 968

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -