Analysis of ATP-Induced Ca2+ Responses at Single Cell Level in Retinal Pigment Epithelium Monolayers.
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review
|Title of host publication||Retinal Degenerative Diseases|
|Subtitle of host publication||Mechanisms and Experimental Therapy|
|Number of pages||6|
|Publication status||Published - 29 Dec 2019|
|Publication type||A4 Article in a conference publication|
|Event||International Symposium on Retinal Degeneration - Killarney, Ireland|
Duration: 3 Sep 2018 → 8 Sep 2018
Conference number: XVIII
|Name||Advances in Experimental Medicine and Biology|
|Conference||International Symposium on Retinal Degeneration|
|Period||3/09/18 → 8/09/18|
Calcium is one of the most important second messengers in cells and thus involved in a variety of physiological processes. In retinal pigment epithelium (RPE), Ca2+ and its ATP-dependent signaling pathways play important roles in the retina maintenance functions. Changes in intracellular Ca2+ concentration can be measured from living cells by Ca2+ imaging. Combining these measurements with quantitative analysis of Ca2+ response properties enables studies of signaling pathways affecting RPE functions. However, robust tools for response analysis from large cell populations are lacking. We developed MATLAB-based analysis tools for single cell level Ca2+ response data recorded from large fields of intact RPE monolayers. The analysis revealed significant heterogeneity in ATP-induced Ca2+ responses inside cell populations regarding magnitude and response kinetics. Further analysis including response grouping and parameter correlations allowed us to characterize the populations at the level of single cells.
- ATP-induced Ca2+ response; Ca2+ imaging; Human embryonic stem cells; Image analysis; Retinal pigment epithelium