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Analysis of ATP-Induced Ca2+ Responses at Single Cell Level in Retinal Pigment Epithelium Monolayers.

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationRetinal Degenerative Diseases
Subtitle of host publicationMechanisms and Experimental Therapy
PublisherSpringer
Pages525-530
Number of pages6
ISBN (Electronic)978-3-030-27378-1
ISBN (Print)978-3-030-27377-4
DOIs
Publication statusPublished - 29 Dec 2019
Publication typeA4 Article in a conference publication
EventInternational Symposium on Retinal Degeneration - Killarney, Ireland
Duration: 3 Sep 20188 Sep 2018
Conference number: XVIII

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1185
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Conference

ConferenceInternational Symposium on Retinal Degeneration
Abbreviated titleRD2018
CountryIreland
CityKillarney
Period3/09/188/09/18

Abstract

Calcium is one of the most important second messengers in cells and thus involved in a variety of physiological processes. In retinal pigment epithelium (RPE), Ca2+ and its ATP-dependent signaling pathways play important roles in the retina maintenance functions. Changes in intracellular Ca2+ concentration can be measured from living cells by Ca2+ imaging. Combining these measurements with quantitative analysis of Ca2+ response properties enables studies of signaling pathways affecting RPE functions. However, robust tools for response analysis from large cell populations are lacking. We developed MATLAB-based analysis tools for single cell level Ca2+ response data recorded from large fields of intact RPE monolayers. The analysis revealed significant heterogeneity in ATP-induced Ca2+ responses inside cell populations regarding magnitude and response kinetics. Further analysis including response grouping and parameter correlations allowed us to characterize the populations at the level of single cells.

Keywords

  • ATP-induced Ca2+ response; Ca2+ imaging; Human embryonic stem cells; Image analysis; Retinal pigment epithelium

Publication forum classification

Field of science, Statistics Finland