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Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.

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Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level. / Palanivel, Suresh; Yli-Harja, Olli; Kandhavelu, Meenakshisundaram.

2014. 88 Paper presented at BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014, .

Research output: Other conference contributionPaper, poster or abstractScientific

Harvard

Palanivel, S, Yli-Harja, O & Kandhavelu, M 2014, 'Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.' Paper presented at BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014, 1/01/14, pp. 88.

APA

Palanivel, S., Yli-Harja, O., & Kandhavelu, M. (2014). Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.. 88. Paper presented at BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014, .

Vancouver

Palanivel S, Yli-Harja O, Kandhavelu M. Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.. 2014. Paper presented at BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014, .

Author

Palanivel, Suresh ; Yli-Harja, Olli ; Kandhavelu, Meenakshisundaram. / Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level. Paper presented at BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014, .1 p.

Bibtex - Download

@conference{8c7f8fe2d48c45e2b823d1158d2d622c,
title = "Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.",
abstract = "G Protein‐Coupled Receptors signal transduction pathways plays crucial role in the many neuronal diseases including Alzheimer’s disease, Parkinson’s disease and Multiple Sclerosis. Recently, GPR17 found to be a unique target for humanneurodegenerative disease. This receptor is typically present in neurons and acts as a classical ligand‐activated GPCR, which responds to uracil nucleotides and cysteinyl‐leukotrienes. GPR17 acts as a mediator in neurotic progression as wellas involved in cell death mechanism in diseased state. Thus, GPR17 is considered as a predetermined therapeutic target. Existence of divergent functions of GPR17 been reported. It was previously reported that activation of GPR17 leads to the adenylyl cyclase inhibition which also results in the increased intracellular calcium concentration. However, detailed molecular mechanism of GPR17 not well‐studied. The central focus of this study is to analyse Ca2+ and cyclic AMP level regulated by GPR17 at single cell level using biosensors and computational biology approaches. For this we optimized the transfection method and single cell imaging protocol which allows us to detect minimal changes in single cell. In the present report we will discuss our preliminary finding on how GPR17 signalling affects Ca2+ and cAMP at single cell level.",
author = "Suresh Palanivel and Olli Yli-Harja and Meenakshisundaram Kandhavelu",
year = "2014",
month = "12",
day = "5",
language = "English",
pages = "88",
note = "BioMediTech Research Day 2014, Tampere, Finland. 5.12.2014 ; Conference date: 01-01-2014",

}

RIS (suitable for import to EndNote) - Download

TY - CONF

T1 - Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.

AU - Palanivel, Suresh

AU - Yli-Harja, Olli

AU - Kandhavelu, Meenakshisundaram

PY - 2014/12/5

Y1 - 2014/12/5

N2 - G Protein‐Coupled Receptors signal transduction pathways plays crucial role in the many neuronal diseases including Alzheimer’s disease, Parkinson’s disease and Multiple Sclerosis. Recently, GPR17 found to be a unique target for humanneurodegenerative disease. This receptor is typically present in neurons and acts as a classical ligand‐activated GPCR, which responds to uracil nucleotides and cysteinyl‐leukotrienes. GPR17 acts as a mediator in neurotic progression as wellas involved in cell death mechanism in diseased state. Thus, GPR17 is considered as a predetermined therapeutic target. Existence of divergent functions of GPR17 been reported. It was previously reported that activation of GPR17 leads to the adenylyl cyclase inhibition which also results in the increased intracellular calcium concentration. However, detailed molecular mechanism of GPR17 not well‐studied. The central focus of this study is to analyse Ca2+ and cyclic AMP level regulated by GPR17 at single cell level using biosensors and computational biology approaches. For this we optimized the transfection method and single cell imaging protocol which allows us to detect minimal changes in single cell. In the present report we will discuss our preliminary finding on how GPR17 signalling affects Ca2+ and cAMP at single cell level.

AB - G Protein‐Coupled Receptors signal transduction pathways plays crucial role in the many neuronal diseases including Alzheimer’s disease, Parkinson’s disease and Multiple Sclerosis. Recently, GPR17 found to be a unique target for humanneurodegenerative disease. This receptor is typically present in neurons and acts as a classical ligand‐activated GPCR, which responds to uracil nucleotides and cysteinyl‐leukotrienes. GPR17 acts as a mediator in neurotic progression as wellas involved in cell death mechanism in diseased state. Thus, GPR17 is considered as a predetermined therapeutic target. Existence of divergent functions of GPR17 been reported. It was previously reported that activation of GPR17 leads to the adenylyl cyclase inhibition which also results in the increased intracellular calcium concentration. However, detailed molecular mechanism of GPR17 not well‐studied. The central focus of this study is to analyse Ca2+ and cyclic AMP level regulated by GPR17 at single cell level using biosensors and computational biology approaches. For this we optimized the transfection method and single cell imaging protocol which allows us to detect minimal changes in single cell. In the present report we will discuss our preliminary finding on how GPR17 signalling affects Ca2+ and cAMP at single cell level.

M3 - Paper, poster or abstract

SP - 88

ER -