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Azopolymer photopatterning for directional control of angiogenesis

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Azopolymer photopatterning for directional control of angiogenesis. / Fedele, Chiara; De Gregorio, Maria; Netti, Paolo A.; Cavalli, Silvia; Attanasio, Chiara.

In: Acta Biomaterialia, Vol. 63, 01.11.2017, p. 317-325.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Fedele, C, De Gregorio, M, Netti, PA, Cavalli, S & Attanasio, C 2017, 'Azopolymer photopatterning for directional control of angiogenesis', Acta Biomaterialia, vol. 63, pp. 317-325. https://doi.org/10.1016/j.actbio.2017.09.022

APA

Fedele, C., De Gregorio, M., Netti, P. A., Cavalli, S., & Attanasio, C. (2017). Azopolymer photopatterning for directional control of angiogenesis. Acta Biomaterialia, 63, 317-325. https://doi.org/10.1016/j.actbio.2017.09.022

Vancouver

Fedele C, De Gregorio M, Netti PA, Cavalli S, Attanasio C. Azopolymer photopatterning for directional control of angiogenesis. Acta Biomaterialia. 2017 Nov 1;63:317-325. https://doi.org/10.1016/j.actbio.2017.09.022

Author

Fedele, Chiara ; De Gregorio, Maria ; Netti, Paolo A. ; Cavalli, Silvia ; Attanasio, Chiara. / Azopolymer photopatterning for directional control of angiogenesis. In: Acta Biomaterialia. 2017 ; Vol. 63. pp. 317-325.

Bibtex - Download

@article{4d4b3e105577415796d3e0c9db9ad837,
title = "Azopolymer photopatterning for directional control of angiogenesis",
abstract = "Understanding cellular behavior in response to microenvironmental stimuli is central to tissue engineering. An increasing number of reports emphasize the high sensitivity of cells to the physical characteristics of the surrounding milieu and in particular, topographical cues. In this work, we investigated the influence of dynamic topographic signal presentation on sprout formation and the possibility to obtain a space–time control over sprouting directionality without growth factors, in order to investigate the contribution of just topography in the angiogenic process. To test our hypothesis, we employed a 3D angiogenesis assay based on the use of spheroids derived from human umbilical vein endothelial cells (HUVECs). We then modulated the in situ presentation of topographical cues during early-stage angiogenesis through real-time photopatterning of an azobenzene-containing polymer, poly (Disperse Red 1 methacrylate) (pDR1m). Pattern inscription on the polymer surface was made using the focused laser of a confocal microscope. We demonstrate that during early-stage angiogenesis, sprouts followed the pattern direction, while spheroid cores acquired a polarized shape. These findings confirmed that sprout directionality was influenced by the photo-inscribed pattern, probably through contact guidance of leader cells, thus validating the proposed platform as a valuable tool for understanding complex processes involved in cell-topography interactions in multicellular systems. Statement of Significance The complex relationship between endothelial cells and the surrounding environment that leads to formation of a newly formed vascular network during tissue repair is currently unknown. We have developed an innovative in vitro platform to study these mechanisms in a space and time controlled fashion simulating what happens during regeneration. In particular, we combine a “smart” surface, namely a polymer film, with a three-dimensional living cell aggregate. The polymer is activated by light through which we can design a path to guide cells toward the formation of a new vessel. Our work lies at the intersection of stimuli-responsive biointerfaces and cell biology and may be particularly inspiring for those interested in designing biomaterial surface related to angiogenesis.",
keywords = "Angiogenesis, Azopolymers, Directional sprouting, Photopatterning, Topographical cues",
author = "Chiara Fedele and {De Gregorio}, Maria and Netti, {Paolo A.} and Silvia Cavalli and Chiara Attanasio",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.actbio.2017.09.022",
language = "English",
volume = "63",
pages = "317--325",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Azopolymer photopatterning for directional control of angiogenesis

AU - Fedele, Chiara

AU - De Gregorio, Maria

AU - Netti, Paolo A.

AU - Cavalli, Silvia

AU - Attanasio, Chiara

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Understanding cellular behavior in response to microenvironmental stimuli is central to tissue engineering. An increasing number of reports emphasize the high sensitivity of cells to the physical characteristics of the surrounding milieu and in particular, topographical cues. In this work, we investigated the influence of dynamic topographic signal presentation on sprout formation and the possibility to obtain a space–time control over sprouting directionality without growth factors, in order to investigate the contribution of just topography in the angiogenic process. To test our hypothesis, we employed a 3D angiogenesis assay based on the use of spheroids derived from human umbilical vein endothelial cells (HUVECs). We then modulated the in situ presentation of topographical cues during early-stage angiogenesis through real-time photopatterning of an azobenzene-containing polymer, poly (Disperse Red 1 methacrylate) (pDR1m). Pattern inscription on the polymer surface was made using the focused laser of a confocal microscope. We demonstrate that during early-stage angiogenesis, sprouts followed the pattern direction, while spheroid cores acquired a polarized shape. These findings confirmed that sprout directionality was influenced by the photo-inscribed pattern, probably through contact guidance of leader cells, thus validating the proposed platform as a valuable tool for understanding complex processes involved in cell-topography interactions in multicellular systems. Statement of Significance The complex relationship between endothelial cells and the surrounding environment that leads to formation of a newly formed vascular network during tissue repair is currently unknown. We have developed an innovative in vitro platform to study these mechanisms in a space and time controlled fashion simulating what happens during regeneration. In particular, we combine a “smart” surface, namely a polymer film, with a three-dimensional living cell aggregate. The polymer is activated by light through which we can design a path to guide cells toward the formation of a new vessel. Our work lies at the intersection of stimuli-responsive biointerfaces and cell biology and may be particularly inspiring for those interested in designing biomaterial surface related to angiogenesis.

AB - Understanding cellular behavior in response to microenvironmental stimuli is central to tissue engineering. An increasing number of reports emphasize the high sensitivity of cells to the physical characteristics of the surrounding milieu and in particular, topographical cues. In this work, we investigated the influence of dynamic topographic signal presentation on sprout formation and the possibility to obtain a space–time control over sprouting directionality without growth factors, in order to investigate the contribution of just topography in the angiogenic process. To test our hypothesis, we employed a 3D angiogenesis assay based on the use of spheroids derived from human umbilical vein endothelial cells (HUVECs). We then modulated the in situ presentation of topographical cues during early-stage angiogenesis through real-time photopatterning of an azobenzene-containing polymer, poly (Disperse Red 1 methacrylate) (pDR1m). Pattern inscription on the polymer surface was made using the focused laser of a confocal microscope. We demonstrate that during early-stage angiogenesis, sprouts followed the pattern direction, while spheroid cores acquired a polarized shape. These findings confirmed that sprout directionality was influenced by the photo-inscribed pattern, probably through contact guidance of leader cells, thus validating the proposed platform as a valuable tool for understanding complex processes involved in cell-topography interactions in multicellular systems. Statement of Significance The complex relationship between endothelial cells and the surrounding environment that leads to formation of a newly formed vascular network during tissue repair is currently unknown. We have developed an innovative in vitro platform to study these mechanisms in a space and time controlled fashion simulating what happens during regeneration. In particular, we combine a “smart” surface, namely a polymer film, with a three-dimensional living cell aggregate. The polymer is activated by light through which we can design a path to guide cells toward the formation of a new vessel. Our work lies at the intersection of stimuli-responsive biointerfaces and cell biology and may be particularly inspiring for those interested in designing biomaterial surface related to angiogenesis.

KW - Angiogenesis

KW - Azopolymers

KW - Directional sprouting

KW - Photopatterning

KW - Topographical cues

U2 - 10.1016/j.actbio.2017.09.022

DO - 10.1016/j.actbio.2017.09.022

M3 - Article

VL - 63

SP - 317

EP - 325

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -