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Behavior of the DPH fluorescence probe in membranes perturbed by drugs

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Behavior of the DPH fluorescence probe in membranes perturbed by drugs. / Poojari, Chetan; Wilkosz, Natalia; Lira, Rafael B.; Dimova, Rumiana; Jurkiewicz, Piotr; Petka, Rafał; Kepczynski, Mariusz; Róg, Tomasz.

In: Chemistry and Physics of Lipids, Vol. 223, 104784, 01.09.2019.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Poojari, C, Wilkosz, N, Lira, RB, Dimova, R, Jurkiewicz, P, Petka, R, Kepczynski, M & Róg, T 2019, 'Behavior of the DPH fluorescence probe in membranes perturbed by drugs' Chemistry and Physics of Lipids, vol. 223, 104784. https://doi.org/10.1016/j.chemphyslip.2019.104784

APA

Poojari, C., Wilkosz, N., Lira, R. B., Dimova, R., Jurkiewicz, P., Petka, R., ... Róg, T. (2019). Behavior of the DPH fluorescence probe in membranes perturbed by drugs. Chemistry and Physics of Lipids, 223, [104784]. https://doi.org/10.1016/j.chemphyslip.2019.104784

Vancouver

Poojari C, Wilkosz N, Lira RB, Dimova R, Jurkiewicz P, Petka R et al. Behavior of the DPH fluorescence probe in membranes perturbed by drugs. Chemistry and Physics of Lipids. 2019 Sep 1;223. 104784. https://doi.org/10.1016/j.chemphyslip.2019.104784

Author

Poojari, Chetan ; Wilkosz, Natalia ; Lira, Rafael B. ; Dimova, Rumiana ; Jurkiewicz, Piotr ; Petka, Rafał ; Kepczynski, Mariusz ; Róg, Tomasz. / Behavior of the DPH fluorescence probe in membranes perturbed by drugs. In: Chemistry and Physics of Lipids. 2019 ; Vol. 223.

Bibtex - Download

@article{748c219b7d93402e9482b377310a923d,
title = "Behavior of the DPH fluorescence probe in membranes perturbed by drugs",
abstract = "1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties of lipid bilayers and cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in the use of DPH to predict the order of lipid acyl chains when the lipid bilayer is doped with itraconazole (ITZ), an antifungal drug. Our steady-state fluorescence anisotropy measurements showed a significant decrease in fluorescence anisotropy of DPH embedded in the ITZ-containing membrane, suggesting a substantial increase in membrane fluidity, which indirectly indicates a decrease in the order of the hydrocarbon chains. This result or its interpretation is in disagreement with the fluorescence recovery after photobleaching measurements and molecular dynamics (MD) simulation data. The results of these experiments and calculations indicate an increase in the hydrocarbon chain order. The MD simulations of the bilayer containing both ITZ and DPH provide explanations for these observations. Apparently, in the presence of the drug, the DPH molecules are pushed deeper into the hydrophobic membrane core below the lipid double bonds, and the probe predominately adopts the orientation of the ITZ molecules that is parallel to the membrane surface, instead of orienting parallel to the lipid acyl chains. For this reason, DPH anisotropy provides information related to the less ordered central region of the membrane rather than reporting the properties of the upper segments of the lipid acyl chains.",
keywords = "1,6-diphenyl-1,3,5-hexatriene, Fluorescence anisotropy, Lipid bilayers, Molecular dynamics simulations",
author = "Chetan Poojari and Natalia Wilkosz and Lira, {Rafael B.} and Rumiana Dimova and Piotr Jurkiewicz and Rafał Petka and Mariusz Kepczynski and Tomasz R{\'o}g",
year = "2019",
month = "9",
day = "1",
doi = "10.1016/j.chemphyslip.2019.104784",
language = "English",
volume = "223",
journal = "Chemistry and Physics of Lipids",
issn = "0009-3084",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Behavior of the DPH fluorescence probe in membranes perturbed by drugs

AU - Poojari, Chetan

AU - Wilkosz, Natalia

AU - Lira, Rafael B.

AU - Dimova, Rumiana

AU - Jurkiewicz, Piotr

AU - Petka, Rafał

AU - Kepczynski, Mariusz

AU - Róg, Tomasz

PY - 2019/9/1

Y1 - 2019/9/1

N2 - 1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties of lipid bilayers and cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in the use of DPH to predict the order of lipid acyl chains when the lipid bilayer is doped with itraconazole (ITZ), an antifungal drug. Our steady-state fluorescence anisotropy measurements showed a significant decrease in fluorescence anisotropy of DPH embedded in the ITZ-containing membrane, suggesting a substantial increase in membrane fluidity, which indirectly indicates a decrease in the order of the hydrocarbon chains. This result or its interpretation is in disagreement with the fluorescence recovery after photobleaching measurements and molecular dynamics (MD) simulation data. The results of these experiments and calculations indicate an increase in the hydrocarbon chain order. The MD simulations of the bilayer containing both ITZ and DPH provide explanations for these observations. Apparently, in the presence of the drug, the DPH molecules are pushed deeper into the hydrophobic membrane core below the lipid double bonds, and the probe predominately adopts the orientation of the ITZ molecules that is parallel to the membrane surface, instead of orienting parallel to the lipid acyl chains. For this reason, DPH anisotropy provides information related to the less ordered central region of the membrane rather than reporting the properties of the upper segments of the lipid acyl chains.

AB - 1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties of lipid bilayers and cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in the use of DPH to predict the order of lipid acyl chains when the lipid bilayer is doped with itraconazole (ITZ), an antifungal drug. Our steady-state fluorescence anisotropy measurements showed a significant decrease in fluorescence anisotropy of DPH embedded in the ITZ-containing membrane, suggesting a substantial increase in membrane fluidity, which indirectly indicates a decrease in the order of the hydrocarbon chains. This result or its interpretation is in disagreement with the fluorescence recovery after photobleaching measurements and molecular dynamics (MD) simulation data. The results of these experiments and calculations indicate an increase in the hydrocarbon chain order. The MD simulations of the bilayer containing both ITZ and DPH provide explanations for these observations. Apparently, in the presence of the drug, the DPH molecules are pushed deeper into the hydrophobic membrane core below the lipid double bonds, and the probe predominately adopts the orientation of the ITZ molecules that is parallel to the membrane surface, instead of orienting parallel to the lipid acyl chains. For this reason, DPH anisotropy provides information related to the less ordered central region of the membrane rather than reporting the properties of the upper segments of the lipid acyl chains.

KW - 1,6-diphenyl-1,3,5-hexatriene

KW - Fluorescence anisotropy

KW - Lipid bilayers

KW - Molecular dynamics simulations

U2 - 10.1016/j.chemphyslip.2019.104784

DO - 10.1016/j.chemphyslip.2019.104784

M3 - Article

VL - 223

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

SN - 0009-3084

M1 - 104784

ER -