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Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells

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Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells. / Ojansivu, Miina; Vanhatupa, Sari; Björkvik, Leena; Häkkänen, Heikki; Kellomäki, Minna; Autio, Reija; Ihalainen, Janne A.; Hupa, Leena; Miettinen, Susanna.

In: Acta Biomaterialia, Vol. 21, 15.07.2015, p. 190-203.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Ojansivu, M, Vanhatupa, S, Björkvik, L, Häkkänen, H, Kellomäki, M, Autio, R, Ihalainen, JA, Hupa, L & Miettinen, S 2015, 'Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells', Acta Biomaterialia, vol. 21, pp. 190-203. https://doi.org/10.1016/j.actbio.2015.04.017

APA

Ojansivu, M., Vanhatupa, S., Björkvik, L., Häkkänen, H., Kellomäki, M., Autio, R., ... Miettinen, S. (2015). Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells. Acta Biomaterialia, 21, 190-203. https://doi.org/10.1016/j.actbio.2015.04.017

Vancouver

Ojansivu M, Vanhatupa S, Björkvik L, Häkkänen H, Kellomäki M, Autio R et al. Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells. Acta Biomaterialia. 2015 Jul 15;21:190-203. https://doi.org/10.1016/j.actbio.2015.04.017

Author

Ojansivu, Miina ; Vanhatupa, Sari ; Björkvik, Leena ; Häkkänen, Heikki ; Kellomäki, Minna ; Autio, Reija ; Ihalainen, Janne A. ; Hupa, Leena ; Miettinen, Susanna. / Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells. In: Acta Biomaterialia. 2015 ; Vol. 21. pp. 190-203.

Bibtex - Download

@article{7936c87ae569487ab7a4af9ecdded40c,
title = "Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells",
abstract = "Bioactive glasses are known for their ability to induce osteogenic differentiation of stem cells. To elucidate the mechanism of the osteoinductivity in more detail, we studied whether ionic extracts prepared from a commercial glass S53P4 and from three experimental glasses (2-06, 1-06 and 3-06) are alone sufficient to induce osteogenic differentiation of human adipose stem cells. Cells were cultured using basic medium or osteogenic medium as extract basis. Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days. At 14 days the mineralization in osteogenic medium extracts was excessive compared to the control. Parallel to the increased mineralization we observed a decrease in the cell amount. Raman and Laser Induced Breakdown Spectroscopy analyses confirmed that the mineral consisted of calcium phosphates. Consistently, the osteogenic medium extracts also increased osteocalcin production and collagen Type-I accumulation in the extracellular matrix at 13 days. Of the four osteogenic medium extracts, 2-06 and 3-06 induced the best responses of osteogenesis. However, regardless of the enhanced mineral formation, alkaline phosphatase activity was not promoted by the extracts. The osteogenic medium extracts could potentially provide a fast and effective way to differentiate human adipose stem cells in vitro.",
keywords = "Bioactive glass, Bone tissue engineering, Mesenchymal stem cell, Mineralization, Osteogenic differentiation",
author = "Miina Ojansivu and Sari Vanhatupa and Leena Bj{\"o}rkvik and Heikki H{\"a}kk{\"a}nen and Minna Kellom{\"a}ki and Reija Autio and Ihalainen, {Janne A.} and Leena Hupa and Susanna Miettinen",
note = "EXT={"}Autio, Reija{"}",
year = "2015",
month = "7",
day = "15",
doi = "10.1016/j.actbio.2015.04.017",
language = "English",
volume = "21",
pages = "190--203",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells

AU - Ojansivu, Miina

AU - Vanhatupa, Sari

AU - Björkvik, Leena

AU - Häkkänen, Heikki

AU - Kellomäki, Minna

AU - Autio, Reija

AU - Ihalainen, Janne A.

AU - Hupa, Leena

AU - Miettinen, Susanna

N1 - EXT="Autio, Reija"

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Bioactive glasses are known for their ability to induce osteogenic differentiation of stem cells. To elucidate the mechanism of the osteoinductivity in more detail, we studied whether ionic extracts prepared from a commercial glass S53P4 and from three experimental glasses (2-06, 1-06 and 3-06) are alone sufficient to induce osteogenic differentiation of human adipose stem cells. Cells were cultured using basic medium or osteogenic medium as extract basis. Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days. At 14 days the mineralization in osteogenic medium extracts was excessive compared to the control. Parallel to the increased mineralization we observed a decrease in the cell amount. Raman and Laser Induced Breakdown Spectroscopy analyses confirmed that the mineral consisted of calcium phosphates. Consistently, the osteogenic medium extracts also increased osteocalcin production and collagen Type-I accumulation in the extracellular matrix at 13 days. Of the four osteogenic medium extracts, 2-06 and 3-06 induced the best responses of osteogenesis. However, regardless of the enhanced mineral formation, alkaline phosphatase activity was not promoted by the extracts. The osteogenic medium extracts could potentially provide a fast and effective way to differentiate human adipose stem cells in vitro.

AB - Bioactive glasses are known for their ability to induce osteogenic differentiation of stem cells. To elucidate the mechanism of the osteoinductivity in more detail, we studied whether ionic extracts prepared from a commercial glass S53P4 and from three experimental glasses (2-06, 1-06 and 3-06) are alone sufficient to induce osteogenic differentiation of human adipose stem cells. Cells were cultured using basic medium or osteogenic medium as extract basis. Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days. At 14 days the mineralization in osteogenic medium extracts was excessive compared to the control. Parallel to the increased mineralization we observed a decrease in the cell amount. Raman and Laser Induced Breakdown Spectroscopy analyses confirmed that the mineral consisted of calcium phosphates. Consistently, the osteogenic medium extracts also increased osteocalcin production and collagen Type-I accumulation in the extracellular matrix at 13 days. Of the four osteogenic medium extracts, 2-06 and 3-06 induced the best responses of osteogenesis. However, regardless of the enhanced mineral formation, alkaline phosphatase activity was not promoted by the extracts. The osteogenic medium extracts could potentially provide a fast and effective way to differentiate human adipose stem cells in vitro.

KW - Bioactive glass

KW - Bone tissue engineering

KW - Mesenchymal stem cell

KW - Mineralization

KW - Osteogenic differentiation

U2 - 10.1016/j.actbio.2015.04.017

DO - 10.1016/j.actbio.2015.04.017

M3 - Article

VL - 21

SP - 190

EP - 203

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -