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Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)54-56
Number of pages3
JournalJournal of Microbiological Methods
Volume114
DOIs
Publication statusPublished - 1 Jul 2015
Publication typeA1 Journal article-refereed

Abstract

We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.

Keywords

  • Antibacterial, Bioluminescence, Cell-based assay, Escherichia coli, Natural products, Screening

Publication forum classification

Field of science, Statistics Finland