Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts
Research output: Contribution to journal › Article › Scientific › peer-review
|Number of pages||3|
|Journal||Journal of Microbiological Methods|
|Publication status||Published - 1 Jul 2015|
|Publication type||A1 Journal article-refereed|
We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.
- Antibacterial, Bioluminescence, Cell-based assay, Escherichia coli, Natural products, Screening