Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts
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Details
| Original language | English |
|---|---|
| Pages (from-to) | 54-56 |
| Number of pages | 3 |
| Journal | Journal of Microbiological Methods |
| Volume | 114 |
| DOIs | |
| Publication status | Published - 1 Jul 2015 |
| Publication type | A1 Journal article-refereed |
Abstract
We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4. h with high sensitivity, leading to significant improvements of the process.
ASJC Scopus subject areas
Keywords
- Antibacterial, Bioluminescence, Cell-based assay, Escherichia coli, Natural products, Screening