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Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

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Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells. / Sorkio, Anni E.; Vuorimaa-Laukkanen, Elina P.; Hakola, Hanna M.; Liang, Huamin; Ujula, Tiina A.; Valle-Delgado, Juan José; Österberg, Monika; Yliperttula, Marjo L.; Skottman, Heli.

In: Biomaterials, Vol. 51, 01.05.2015, p. 257-269.

Research output: Contribution to journalArticleScientificpeer-review

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Sorkio, AE, Vuorimaa-Laukkanen, EP, Hakola, HM, Liang, H, Ujula, TA, Valle-Delgado, JJ, Österberg, M, Yliperttula, ML & Skottman, H 2015, 'Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells', Biomaterials, vol. 51, pp. 257-269. https://doi.org/10.1016/j.biomaterials.2015.02.005

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Sorkio, Anni E. ; Vuorimaa-Laukkanen, Elina P. ; Hakola, Hanna M. ; Liang, Huamin ; Ujula, Tiina A. ; Valle-Delgado, Juan José ; Österberg, Monika ; Yliperttula, Marjo L. ; Skottman, Heli. / Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells. In: Biomaterials. 2015 ; Vol. 51. pp. 257-269.

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@article{bce01c6930e647c8888d0db11ce751a2,
title = "Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells",
abstract = "The environmental cues received by the cells from synthetic substrates invitro are very different from those they receive invivo. In this study, we applied the Langmuir-Schaefer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.",
keywords = "Biomimetic material, Collagen structure, Human embryonic stem cell, Langmuir Blodgett film, Retina, Retinal pigment epithelial cell",
author = "Sorkio, {Anni E.} and Vuorimaa-Laukkanen, {Elina P.} and Hakola, {Hanna M.} and Huamin Liang and Ujula, {Tiina A.} and Valle-Delgado, {Juan Jos{\'e}} and Monika {\"O}sterberg and Yliperttula, {Marjo L.} and Heli Skottman",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.biomaterials.2015.02.005",
language = "English",
volume = "51",
pages = "257--269",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Biomimetic collagen I and IV double layer Langmuir-Schaefer films asmicroenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

AU - Sorkio, Anni E.

AU - Vuorimaa-Laukkanen, Elina P.

AU - Hakola, Hanna M.

AU - Liang, Huamin

AU - Ujula, Tiina A.

AU - Valle-Delgado, Juan José

AU - Österberg, Monika

AU - Yliperttula, Marjo L.

AU - Skottman, Heli

PY - 2015/5/1

Y1 - 2015/5/1

N2 - The environmental cues received by the cells from synthetic substrates invitro are very different from those they receive invivo. In this study, we applied the Langmuir-Schaefer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.

AB - The environmental cues received by the cells from synthetic substrates invitro are very different from those they receive invivo. In this study, we applied the Langmuir-Schaefer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.

KW - Biomimetic material

KW - Collagen structure

KW - Human embryonic stem cell

KW - Langmuir Blodgett film

KW - Retina

KW - Retinal pigment epithelial cell

UR - http://www.scopus.com/inward/record.url?scp=84924859980&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2015.02.005

DO - 10.1016/j.biomaterials.2015.02.005

M3 - Article

VL - 51

SP - 257

EP - 269

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -