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Biophysics of lipid bilayers containing oxidatively modified phospholipids: Insights from fluorescence and EPR experiments and from MD simulations

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Biophysics of lipid bilayers containing oxidatively modified phospholipids : Insights from fluorescence and EPR experiments and from MD simulations. / Jurkiewicz, Piotr; Olzyńska, Agnieszka; Cwiklik, Lukasz; Conte, Elena; Jungwirth, Pavel; Megli, Francesco M.; Hof, Martin.

In: Biochimica et Biophysica Acta: Biomembranes, Vol. 1818, No. 10, 10.2012, p. 2388-2402.

Research output: Contribution to journalReview ArticleScientificpeer-review

Harvard

Jurkiewicz, P, Olzyńska, A, Cwiklik, L, Conte, E, Jungwirth, P, Megli, FM & Hof, M 2012, 'Biophysics of lipid bilayers containing oxidatively modified phospholipids: Insights from fluorescence and EPR experiments and from MD simulations', Biochimica et Biophysica Acta: Biomembranes, vol. 1818, no. 10, pp. 2388-2402. https://doi.org/10.1016/j.bbamem.2012.05.020

APA

Jurkiewicz, P., Olzyńska, A., Cwiklik, L., Conte, E., Jungwirth, P., Megli, F. M., & Hof, M. (2012). Biophysics of lipid bilayers containing oxidatively modified phospholipids: Insights from fluorescence and EPR experiments and from MD simulations. Biochimica et Biophysica Acta: Biomembranes, 1818(10), 2388-2402. https://doi.org/10.1016/j.bbamem.2012.05.020

Vancouver

Jurkiewicz P, Olzyńska A, Cwiklik L, Conte E, Jungwirth P, Megli FM et al. Biophysics of lipid bilayers containing oxidatively modified phospholipids: Insights from fluorescence and EPR experiments and from MD simulations. Biochimica et Biophysica Acta: Biomembranes. 2012 Oct;1818(10):2388-2402. https://doi.org/10.1016/j.bbamem.2012.05.020

Author

Jurkiewicz, Piotr ; Olzyńska, Agnieszka ; Cwiklik, Lukasz ; Conte, Elena ; Jungwirth, Pavel ; Megli, Francesco M. ; Hof, Martin. / Biophysics of lipid bilayers containing oxidatively modified phospholipids : Insights from fluorescence and EPR experiments and from MD simulations. In: Biochimica et Biophysica Acta: Biomembranes. 2012 ; Vol. 1818, No. 10. pp. 2388-2402.

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@article{6ee8cc6f2eac406a90bb2a99e2ab89f7,
title = "Biophysics of lipid bilayers containing oxidatively modified phospholipids: Insights from fluorescence and EPR experiments and from MD simulations",
abstract = "This review focuses on the influence of oxidized phosphatidylcholines (oxPCs) on the biophysical properties of model membranes and is limited to fluorescence, EPR, and MD studies. OxPCs are divided into two classes: A) hydroxy- or hydroperoxy-dieonyl phospatidylcholines, B) phospatidylcholines with oxidized and truncated chains with either aldehyde or carboxylic group. It was shown that the presence of the investigated oxPCs in phospholipid model membranes may have the following consequences: 1) decrease of the lipid order, 2) lowering of phase transition temperatures, 3) lateral expansion and thinning of the bilayer, 4) alterations of bilayer hydration profiles, 5) increased lipid mobility, 6) augmented flip-flop, 7) influence on the lateral phase organisation, and 8) promotion of water defects and, under extreme conditions (i.e. high concentrations of class B oxPCs), disintegration of the bilayer. The effects of class A oxPCs appear to be more moderate than those observed or predicted for class B. Many of the abovementioned findings are related to the ability of the oxidized chains of certain oxPCs to reorient toward the water phase. Some of the effects appear to be moderated by the presence of cholesterol. Although those biophysical alternations are found at oxPC concentrations higher than the total oxPC concentrations found under physiological conditions, certain organelles may reach such elevated oxPC concentrations locally. It is a challenge for the future to correlate the biophysics of oxidized phospholipids to metabolic studies in order to define the significance of the findings presented herein for pathophysiology. This article is part of a Special Issue entitled: Oxidized phospholipids - their properties and interactions with proteins.",
keywords = "EPR, Fluorescence, Liposome, Molecular dynamics, Oxidized phospholipids",
author = "Piotr Jurkiewicz and Agnieszka Olzyńska and Lukasz Cwiklik and Elena Conte and Pavel Jungwirth and Megli, {Francesco M.} and Martin Hof",
year = "2012",
month = "10",
doi = "10.1016/j.bbamem.2012.05.020",
language = "English",
volume = "1818",
pages = "2388--2402",
journal = "Biochimica et Biophysica Acta: Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "10",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Biophysics of lipid bilayers containing oxidatively modified phospholipids

T2 - Insights from fluorescence and EPR experiments and from MD simulations

AU - Jurkiewicz, Piotr

AU - Olzyńska, Agnieszka

AU - Cwiklik, Lukasz

AU - Conte, Elena

AU - Jungwirth, Pavel

AU - Megli, Francesco M.

AU - Hof, Martin

PY - 2012/10

Y1 - 2012/10

N2 - This review focuses on the influence of oxidized phosphatidylcholines (oxPCs) on the biophysical properties of model membranes and is limited to fluorescence, EPR, and MD studies. OxPCs are divided into two classes: A) hydroxy- or hydroperoxy-dieonyl phospatidylcholines, B) phospatidylcholines with oxidized and truncated chains with either aldehyde or carboxylic group. It was shown that the presence of the investigated oxPCs in phospholipid model membranes may have the following consequences: 1) decrease of the lipid order, 2) lowering of phase transition temperatures, 3) lateral expansion and thinning of the bilayer, 4) alterations of bilayer hydration profiles, 5) increased lipid mobility, 6) augmented flip-flop, 7) influence on the lateral phase organisation, and 8) promotion of water defects and, under extreme conditions (i.e. high concentrations of class B oxPCs), disintegration of the bilayer. The effects of class A oxPCs appear to be more moderate than those observed or predicted for class B. Many of the abovementioned findings are related to the ability of the oxidized chains of certain oxPCs to reorient toward the water phase. Some of the effects appear to be moderated by the presence of cholesterol. Although those biophysical alternations are found at oxPC concentrations higher than the total oxPC concentrations found under physiological conditions, certain organelles may reach such elevated oxPC concentrations locally. It is a challenge for the future to correlate the biophysics of oxidized phospholipids to metabolic studies in order to define the significance of the findings presented herein for pathophysiology. This article is part of a Special Issue entitled: Oxidized phospholipids - their properties and interactions with proteins.

AB - This review focuses on the influence of oxidized phosphatidylcholines (oxPCs) on the biophysical properties of model membranes and is limited to fluorescence, EPR, and MD studies. OxPCs are divided into two classes: A) hydroxy- or hydroperoxy-dieonyl phospatidylcholines, B) phospatidylcholines with oxidized and truncated chains with either aldehyde or carboxylic group. It was shown that the presence of the investigated oxPCs in phospholipid model membranes may have the following consequences: 1) decrease of the lipid order, 2) lowering of phase transition temperatures, 3) lateral expansion and thinning of the bilayer, 4) alterations of bilayer hydration profiles, 5) increased lipid mobility, 6) augmented flip-flop, 7) influence on the lateral phase organisation, and 8) promotion of water defects and, under extreme conditions (i.e. high concentrations of class B oxPCs), disintegration of the bilayer. The effects of class A oxPCs appear to be more moderate than those observed or predicted for class B. Many of the abovementioned findings are related to the ability of the oxidized chains of certain oxPCs to reorient toward the water phase. Some of the effects appear to be moderated by the presence of cholesterol. Although those biophysical alternations are found at oxPC concentrations higher than the total oxPC concentrations found under physiological conditions, certain organelles may reach such elevated oxPC concentrations locally. It is a challenge for the future to correlate the biophysics of oxidized phospholipids to metabolic studies in order to define the significance of the findings presented herein for pathophysiology. This article is part of a Special Issue entitled: Oxidized phospholipids - their properties and interactions with proteins.

KW - EPR

KW - Fluorescence

KW - Liposome

KW - Molecular dynamics

KW - Oxidized phospholipids

UR - http://www.scopus.com/inward/record.url?scp=84863984081&partnerID=8YFLogxK

U2 - 10.1016/j.bbamem.2012.05.020

DO - 10.1016/j.bbamem.2012.05.020

M3 - Review Article

VL - 1818

SP - 2388

EP - 2402

JO - Biochimica et Biophysica Acta: Biomembranes

JF - Biochimica et Biophysica Acta: Biomembranes

SN - 0005-2736

IS - 10

ER -