Tampere University of Technology

TUTCRIS Research Portal

Cell culture chamber with gas supply for prolonged recording of human neuronal cells on microelectrode array

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalJournal of Neuroscience Methods
Publication statusPublished - 15 Mar 2017
Publication typeA1 Journal article-refereed


Background Typically, live cell analyses are performed outside an incubator in an ambient air, where the lack of sufficient CO2 supply results in a fast change of pH and the high evaporation causes concentration drifts in the culture medium. That limits the experiment time for tens of minutes. In many applications, e.g. in neurotoxicity studies, a prolonged measurement of extracellular activity is, however, essential. New method We demonstrate a simple cell culture chamber that enables stable culture conditions during prolonged extracellular recordings on a microelectrode array (MEA) outside an incubator. The proposed chamber consists of a gas permeable silicone structure that enables gas transfer into the chamber. Results We show that the culture chamber supports the growth of the human embryonic stem cell (hESC)-derived neurons both inside and outside an incubator. The structure provides very low evaporation, stable pH and osmolarity, and maintains strong signaling of hESC-derived neuronal networks over three-day MEA experiments. Comparison with existing methods Existing systems are typically complex including continuous perfusion of medium or relatively large amount of gas to supply. The proposed chamber requires only a supply of very low flow rate (1.5 ml/min) of non-humidified 5% CO2 gas. Utilizing dry gas supply makes the proposed chamber simple to use. Conclusion Using the proposed culture structure on top of MEA, we can maintain hESC-derived neural networks over three days outside an incubator. Technically, the structure requires very low flow rate of dry gas supporting, however, low evaporation and maintaining the pH of the culture.

ASJC Scopus subject areas


  • Cell culture environment, Human embryonic stem cells, Long-term culture, MEA, Neuronal cells, Osmolarity, PDMS, pH

Publication forum classification

Field of science, Statistics Finland

Downloads statistics

No data available