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Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)481-490
Number of pages10
JournalBiotechnology and Bioengineering
Volume108
Issue number3
DOIs
Publication statusPublished - Mar 2011
Publication typeA1 Journal article-refereed

Abstract

Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490.

Keywords

  • Avidin-biotin, Nanobiotechnology, Protein engineering, Thermodynamics, X-ray structure