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Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure

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Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure. / Määttä, Juha A.E.; Eisenberg-Domovich, Yael; Nordlund, Henri R.; Hayouka, Ruchama; Kulomaa, Markku S.; Livnah, Oded; Hytönen, Vesa P.

In: Biotechnology and Bioengineering, Vol. 108, No. 3, 03.2011, p. 481-490.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Määttä, JAE, Eisenberg-Domovich, Y, Nordlund, HR, Hayouka, R, Kulomaa, MS, Livnah, O & Hytönen, VP 2011, 'Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure', Biotechnology and Bioengineering, vol. 108, no. 3, pp. 481-490. https://doi.org/10.1002/bit.22962

APA

Määttä, J. A. E., Eisenberg-Domovich, Y., Nordlund, H. R., Hayouka, R., Kulomaa, M. S., Livnah, O., & Hytönen, V. P. (2011). Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure. Biotechnology and Bioengineering, 108(3), 481-490. https://doi.org/10.1002/bit.22962

Vancouver

Määttä JAE, Eisenberg-Domovich Y, Nordlund HR, Hayouka R, Kulomaa MS, Livnah O et al. Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure. Biotechnology and Bioengineering. 2011 Mar;108(3):481-490. https://doi.org/10.1002/bit.22962

Author

Määttä, Juha A.E. ; Eisenberg-Domovich, Yael ; Nordlund, Henri R. ; Hayouka, Ruchama ; Kulomaa, Markku S. ; Livnah, Oded ; Hytönen, Vesa P. / Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure. In: Biotechnology and Bioengineering. 2011 ; Vol. 108, No. 3. pp. 481-490.

Bibtex - Download

@article{88122ed75e4a4714abaf028cf05f6758,
title = "Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure",
abstract = "Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490.",
keywords = "Avidin-biotin, Nanobiotechnology, Protein engineering, Thermodynamics, X-ray structure",
author = "M{\"a}{\"a}tt{\"a}, {Juha A.E.} and Yael Eisenberg-Domovich and Nordlund, {Henri R.} and Ruchama Hayouka and Kulomaa, {Markku S.} and Oded Livnah and Hyt{\"o}nen, {Vesa P.}",
year = "2011",
month = "3",
doi = "10.1002/bit.22962",
language = "English",
volume = "108",
pages = "481--490",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure

AU - Määttä, Juha A.E.

AU - Eisenberg-Domovich, Yael

AU - Nordlund, Henri R.

AU - Hayouka, Ruchama

AU - Kulomaa, Markku S.

AU - Livnah, Oded

AU - Hytönen, Vesa P.

PY - 2011/3

Y1 - 2011/3

N2 - Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490.

AB - Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490.

KW - Avidin-biotin

KW - Nanobiotechnology

KW - Protein engineering

KW - Thermodynamics

KW - X-ray structure

UR - http://www.scopus.com/inward/record.url?scp=78751523876&partnerID=8YFLogxK

U2 - 10.1002/bit.22962

DO - 10.1002/bit.22962

M3 - Article

VL - 108

SP - 481

EP - 490

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 3

ER -