Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols
Research output: Contribution to journal › Article › Scientific › peer-review
|Number of pages||12|
|Journal||Free Radical Biology and Medicine|
|Publication status||Published - 1 Jul 2015|
|Publication type||A1 Journal article-refereed|
The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.
- Phospholipid bilayers, Oxysterols, Molecular dynamics simulations, DPH anisotropy, NMR measurements, Laurdan fluorescence, Liposomes, Tilt modulus, FLUORESCENCE SOLVENT RELAXATION, MOLECULAR-DYNAMICS METHOD, MODEL MEMBRANES, FOURIER TRANSFORMATION, POTENTIAL FUNCTIONS, SOLVATION DYNAMICS, BENDING RIGIDITY, ORDER PARAMETERS, BILAYERS, PROTEINS