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Cholesterol under oxidative stress: How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

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Details

Original languageEnglish
Pages (from-to)30-41
Number of pages12
JournalFree Radical Biology and Medicine
Volume84
DOIs
Publication statusPublished - 1 Jul 2015
Publication typeA1 Journal article-refereed

Abstract

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized - tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

ASJC Scopus subject areas

Keywords

  • Phospholipid bilayers, Oxysterols, Molecular dynamics simulations, DPH anisotropy, NMR measurements, Laurdan fluorescence, Liposomes, Tilt modulus, FLUORESCENCE SOLVENT RELAXATION, MOLECULAR-DYNAMICS METHOD, MODEL MEMBRANES, FOURIER TRANSFORMATION, POTENTIAL FUNCTIONS, SOLVATION DYNAMICS, BENDING RIGIDITY, ORDER PARAMETERS, BILAYERS, PROTEINS

Publication forum classification

Field of science, Statistics Finland