Chromatin organization regulates viral egress dynamics

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Chromatin organization regulates viral egress dynamics. / Aho, Vesa; Myllys, Markko; Ruokolainen, Visa; Hakanen, Satu; Mäntylä, Elina; Virtanen, Jori; Hukkanen, Veijo; Kühn, Thomas; Timonen, Jussi; Mattila, Keijo; Larabell, Carolyn A.; Vihinen-Ranta, Maija.

In: Scientific Reports, Vol. 7, No. 1, 3692, 01.12.2017.

Research output: Contribution to journal › Article › Scientific › peer-review

Harvard

Aho, V, Myllys, M, Ruokolainen, V, Hakanen, S, Mäntylä, E, Virtanen, J, Hukkanen, V, Kühn, T, Timonen, J, Mattila, K, Larabell, CA & Vihinen-Ranta, M 2017, 'Chromatin organization regulates viral egress dynamics', Scientific Reports, vol. 7, no. 1, 3692. https://doi.org/10.1038/s41598-017-03630-y

APA

Aho, V., Myllys, M., Ruokolainen, V., Hakanen, S., Mäntylä, E., Virtanen, J., ... Vihinen-Ranta, M. (2017). Chromatin organization regulates viral egress dynamics. Scientific Reports, 7(1), [3692]. https://doi.org/10.1038/s41598-017-03630-y

Vancouver

Aho V, Myllys M, Ruokolainen V, Hakanen S, Mäntylä E, Virtanen J et al. Chromatin organization regulates viral egress dynamics. Scientific Reports. 2017 Dec 1;7(1). 3692. https://doi.org/10.1038/s41598-017-03630-y

Author

Aho, Vesa ; Myllys, Markko ; Ruokolainen, Visa ; Hakanen, Satu ; Mäntylä, Elina ; Virtanen, Jori ; Hukkanen, Veijo ; Kühn, Thomas ; Timonen, Jussi ; Mattila, Keijo ; Larabell, Carolyn A. ; Vihinen-Ranta, Maija. / Chromatin organization regulates viral egress dynamics. In: Scientific Reports. 2017 ; Vol. 7, No. 1.

Bibtex - Download

@article{3e6bdbbe1f3747bf942b3c7302618102,

title = "Chromatin organization regulates viral egress dynamics",

abstract = "Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.",

author = "Vesa Aho and Markko Myllys and Visa Ruokolainen and Satu Hakanen and Elina M{\"a}ntyl{\"a} and Jori Virtanen and Veijo Hukkanen and Thomas K{\"u}hn and Jussi Timonen and Keijo Mattila and Larabell, {Carolyn A.} and Maija Vihinen-Ranta",

year = "2017",

month = "12",

day = "1",

doi = "10.1038/s41598-017-03630-y",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Chromatin organization regulates viral egress dynamics

AU - Aho, Vesa

AU - Myllys, Markko

AU - Ruokolainen, Visa

AU - Hakanen, Satu

AU - Mäntylä, Elina

AU - Virtanen, Jori

AU - Hukkanen, Veijo

AU - Kühn, Thomas

AU - Timonen, Jussi

AU - Mattila, Keijo

AU - Larabell, Carolyn A.

AU - Vihinen-Ranta, Maija

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.

AB - Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.

U2 - 10.1038/s41598-017-03630-y

DO - 10.1038/s41598-017-03630-y

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3692

ER -

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