Tampere University of Technology

TUTCRIS Research Portal

Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films

Research output: Contribution to journalArticleScientificpeer-review

Standard

Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films. / Rebelo Calejo, Teresa; Vuorenpää, Hanna; Vuorimaa-Laukkanen, Elina; Kallio, Pasi; Aalto-Setälä, Katriina; Miettinen, Susanna; Skottman, Heli; Kellomäki, Minna; Juuti-Uusitalo, Kati.

In: Acta Biomaterialia, Vol. 101, 2020, p. 327-343.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

APA

Vancouver

Author

Rebelo Calejo, Teresa ; Vuorenpää, Hanna ; Vuorimaa-Laukkanen, Elina ; Kallio, Pasi ; Aalto-Setälä, Katriina ; Miettinen, Susanna ; Skottman, Heli ; Kellomäki, Minna ; Juuti-Uusitalo, Kati. / Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films. In: Acta Biomaterialia. 2020 ; Vol. 101. pp. 327-343.

Bibtex - Download

@article{dbcbec1f7fd042e8823ebfb38e5c9975,
title = "Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films",
abstract = "In vitro cell culture models representing the physiological and pathological features of the outer retina are urgently needed. Artificial tissue replacements for patients suffering from degenerative retinal diseases are similarly in great demand. Here, we developed a co-culture system based solely on the use of human induced pluripotent stem cell (hiPSC)-derived cells. For the first time, hiPSC-derived retinal pigment epithelium (RPE) and endothelial cells (EC) were cultured on opposite sides of porous polylactide substrates prepared by breath figures (BF), where both surfaces had been collagen-coated by Langmuir–Schaefer (LS) technology. Small modifications of casting conditions during material preparation allowed the production of free-standing materials with distinct porosity, wettability and ion diffusion capacity. Complete pore coverage was achieved by the collagen coating procedure, resulting in a detectable nanoscale topography. Primary retinal endothelial cells (ACBRI181) and umbilical cord vein endothelial cells (hUVEC) were utilised as EC references. Mono-cultures of all ECs were prepared for comparison. All tested materials supported cell attachment and growth. In mono-culture, properties of the materials had a major effect on the growth of all ECs. In co-culture, the presence of hiPSC-RPE affected the primary ECs more significantly than hiPSC-EC. In consistency, hiPSC-RPE were also less affected by hiPSC-EC than by the primary ECs. Finally, our results show that the modulation of the porosity of the materials can promote or prevent EC migration. In short, we showed that the behaviour of the cells is highly dependent on the three main variables of the study: the presence of a second cell type in co-culture, the source of endothelial cells and the biomaterial properties. The combination of BF and LS methodologies is a powerful strategy to develop thin but stable materials enabling cell growth and modulation of cell-cell contact. Statement of significance: Artificial blood-retinal barriers (BRB), mimicking the interface at the back of the eye, are urgently needed as physiological and disease models, and for tissue transplantation targeting patients suffering from degenerative retinal diseases. Here, we developed a new co-culture model based on thin, biodegradable porous films, coated on both sides with collagen, one of the main components of the natural BRB, and cultivated endothelial and retinal pigment epithelial cells on opposite sides of the films, forming a three-layer structure. Importantly, our hiPSC-EC and hiPSC-RPE co-culture model is the first to exclusively use human induced pluripotent stem cells as cell source, which have been widely regarded as an practical candidate for therapeutic applications in regenerative medicine.",
keywords = "Breath figures, Co-culture, hiPSC-endothelial cells, hiPSC-RPE, Polylactide",
author = "{Rebelo Calejo}, Teresa and Hanna Vuorenp{\"a}{\"a} and Elina Vuorimaa-Laukkanen and Pasi Kallio and Katriina Aalto-Set{\"a}l{\"a} and Susanna Miettinen and Heli Skottman and Minna Kellom{\"a}ki and Kati Juuti-Uusitalo",
note = "INT=BMTE,{"}Saari, Jaakko{"} INT=BMTE,{"}Vuorenp{\"a}{\"a}, Hanna{"} INT=BMTE,{"}Aalto-Set{\"a}l{\"a}, Katriina{"} INT=BMTE,{"}Miettinen, Susanna{"} INT=BMTE,{"}Skottman, Heli{"} INT=BMTE,{"}Juuti-Uusitalo, Kati{"}",
year = "2020",
doi = "10.1016/j.actbio.2019.11.002",
language = "English",
volume = "101",
pages = "327--343",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films

AU - Rebelo Calejo, Teresa

AU - Vuorenpää, Hanna

AU - Vuorimaa-Laukkanen, Elina

AU - Kallio, Pasi

AU - Aalto-Setälä, Katriina

AU - Miettinen, Susanna

AU - Skottman, Heli

AU - Kellomäki, Minna

AU - Juuti-Uusitalo, Kati

N1 - INT=BMTE,"Saari, Jaakko" INT=BMTE,"Vuorenpää, Hanna" INT=BMTE,"Aalto-Setälä, Katriina" INT=BMTE,"Miettinen, Susanna" INT=BMTE,"Skottman, Heli" INT=BMTE,"Juuti-Uusitalo, Kati"

PY - 2020

Y1 - 2020

N2 - In vitro cell culture models representing the physiological and pathological features of the outer retina are urgently needed. Artificial tissue replacements for patients suffering from degenerative retinal diseases are similarly in great demand. Here, we developed a co-culture system based solely on the use of human induced pluripotent stem cell (hiPSC)-derived cells. For the first time, hiPSC-derived retinal pigment epithelium (RPE) and endothelial cells (EC) were cultured on opposite sides of porous polylactide substrates prepared by breath figures (BF), where both surfaces had been collagen-coated by Langmuir–Schaefer (LS) technology. Small modifications of casting conditions during material preparation allowed the production of free-standing materials with distinct porosity, wettability and ion diffusion capacity. Complete pore coverage was achieved by the collagen coating procedure, resulting in a detectable nanoscale topography. Primary retinal endothelial cells (ACBRI181) and umbilical cord vein endothelial cells (hUVEC) were utilised as EC references. Mono-cultures of all ECs were prepared for comparison. All tested materials supported cell attachment and growth. In mono-culture, properties of the materials had a major effect on the growth of all ECs. In co-culture, the presence of hiPSC-RPE affected the primary ECs more significantly than hiPSC-EC. In consistency, hiPSC-RPE were also less affected by hiPSC-EC than by the primary ECs. Finally, our results show that the modulation of the porosity of the materials can promote or prevent EC migration. In short, we showed that the behaviour of the cells is highly dependent on the three main variables of the study: the presence of a second cell type in co-culture, the source of endothelial cells and the biomaterial properties. The combination of BF and LS methodologies is a powerful strategy to develop thin but stable materials enabling cell growth and modulation of cell-cell contact. Statement of significance: Artificial blood-retinal barriers (BRB), mimicking the interface at the back of the eye, are urgently needed as physiological and disease models, and for tissue transplantation targeting patients suffering from degenerative retinal diseases. Here, we developed a new co-culture model based on thin, biodegradable porous films, coated on both sides with collagen, one of the main components of the natural BRB, and cultivated endothelial and retinal pigment epithelial cells on opposite sides of the films, forming a three-layer structure. Importantly, our hiPSC-EC and hiPSC-RPE co-culture model is the first to exclusively use human induced pluripotent stem cells as cell source, which have been widely regarded as an practical candidate for therapeutic applications in regenerative medicine.

AB - In vitro cell culture models representing the physiological and pathological features of the outer retina are urgently needed. Artificial tissue replacements for patients suffering from degenerative retinal diseases are similarly in great demand. Here, we developed a co-culture system based solely on the use of human induced pluripotent stem cell (hiPSC)-derived cells. For the first time, hiPSC-derived retinal pigment epithelium (RPE) and endothelial cells (EC) were cultured on opposite sides of porous polylactide substrates prepared by breath figures (BF), where both surfaces had been collagen-coated by Langmuir–Schaefer (LS) technology. Small modifications of casting conditions during material preparation allowed the production of free-standing materials with distinct porosity, wettability and ion diffusion capacity. Complete pore coverage was achieved by the collagen coating procedure, resulting in a detectable nanoscale topography. Primary retinal endothelial cells (ACBRI181) and umbilical cord vein endothelial cells (hUVEC) were utilised as EC references. Mono-cultures of all ECs were prepared for comparison. All tested materials supported cell attachment and growth. In mono-culture, properties of the materials had a major effect on the growth of all ECs. In co-culture, the presence of hiPSC-RPE affected the primary ECs more significantly than hiPSC-EC. In consistency, hiPSC-RPE were also less affected by hiPSC-EC than by the primary ECs. Finally, our results show that the modulation of the porosity of the materials can promote or prevent EC migration. In short, we showed that the behaviour of the cells is highly dependent on the three main variables of the study: the presence of a second cell type in co-culture, the source of endothelial cells and the biomaterial properties. The combination of BF and LS methodologies is a powerful strategy to develop thin but stable materials enabling cell growth and modulation of cell-cell contact. Statement of significance: Artificial blood-retinal barriers (BRB), mimicking the interface at the back of the eye, are urgently needed as physiological and disease models, and for tissue transplantation targeting patients suffering from degenerative retinal diseases. Here, we developed a new co-culture model based on thin, biodegradable porous films, coated on both sides with collagen, one of the main components of the natural BRB, and cultivated endothelial and retinal pigment epithelial cells on opposite sides of the films, forming a three-layer structure. Importantly, our hiPSC-EC and hiPSC-RPE co-culture model is the first to exclusively use human induced pluripotent stem cells as cell source, which have been widely regarded as an practical candidate for therapeutic applications in regenerative medicine.

KW - Breath figures

KW - Co-culture

KW - hiPSC-endothelial cells

KW - hiPSC-RPE

KW - Polylactide

U2 - 10.1016/j.actbio.2019.11.002

DO - 10.1016/j.actbio.2019.11.002

M3 - Article

VL - 101

SP - 327

EP - 343

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -