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Collagen-immobilized polyimide membranes for retinal pigment epithelial cell adherence and proliferation

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Details

Original languageEnglish
JournalCogent Chemistry
Volume3
Issue number1
DOIs
Publication statusPublished - 6 Mar 2017
Publication typeA1 Journal article-refereed

Abstract

Degenerative retinal diseases are a leading cause of visual loss and irreversible blindness, particularly in the developed world. Retinal pigment cell (RPE) transplantation is nowadays considered the most promising therapeutic approach for certain retinal diseases, and the presence of a supportive scaffold has been considered essential to ensure the success of the implant. In this work, collagen IV was covalently immobilized to the surface of polyimide membranes, with the purpose of developing scaffold materials for RPE cell culture. The covalent modification method involved four steps: argon-plasma treatment, acrylic acid graft polymerization, surface activation, and finally immobilization of collagen type IV. Collagen-modified membranes did not become more rough but became significantly more hydrophilic than the unmodified and dip-coated controls. ARPE-19 cell morphology and attachment were studied by immunofluorescence staining and confocal microscopy. Covalently modified surfaces showed cell attachment and cell properties comparable to the uncoated and dip-coated controls. This work demonstrated the potential of collagen IV-immobilized polyimide membranes as substrates for the growth of ARPE-19 cells.

Keywords

  • Polyimide, Retinal pigment epithelial cell, SURFACE MODIFICATION, tissue engineering

Publication forum classification

Field of science, Statistics Finland

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