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Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery

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Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery. / Zorzi, Giovanni K.; Párraga, Jenny E.; Seijo, Begoña; Sanchez, Alejandro.

In: Colloids and Surfaces B: Biointerfaces, Vol. 135, 01.11.2015, p. 533-541.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Zorzi, GK, Párraga, JE, Seijo, B & Sanchez, A 2015, 'Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery', Colloids and Surfaces B: Biointerfaces, vol. 135, pp. 533-541. https://doi.org/10.1016/j.colsurfb.2015.08.008

APA

Zorzi, G. K., Párraga, J. E., Seijo, B., & Sanchez, A. (2015). Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery. Colloids and Surfaces B: Biointerfaces, 135, 533-541. https://doi.org/10.1016/j.colsurfb.2015.08.008

Vancouver

Zorzi GK, Párraga JE, Seijo B, Sanchez A. Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery. Colloids and Surfaces B: Biointerfaces. 2015 Nov 1;135:533-541. https://doi.org/10.1016/j.colsurfb.2015.08.008

Author

Zorzi, Giovanni K. ; Párraga, Jenny E. ; Seijo, Begoña ; Sanchez, Alejandro. / Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery. In: Colloids and Surfaces B: Biointerfaces. 2015 ; Vol. 135. pp. 533-541.

Bibtex - Download

@article{01fb535e0bcf42a198ab057f259ec689,
title = "Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery",
abstract = "Cationized polymers have been proposed as transfection agents for gene therapy. The present work aims to improve the understanding of the potential use of different cationized proteins (atelocollagen, albumin and gelatin) as nanoparticle components and to investigate the possibility of modulating the physicochemical properties of the resulting nanoparticle carriers by selecting specific protein characteristics in an attempt to improve current ocular gene-delivery approaches. The toxicity profiles, as well as internalization and transfection efficiency, of the developed nanoparticles can be modulated by modifying the molecular weight of the selected protein and the amine used for cationization. The most promising systems are nanoparticles based on intermediate molecular weight gelatin cationized with the endogenous amine spermine, which exhibit an adequate toxicological profile, as well as effective association and protection of pDNA or siRNA molecules, thereby resulting in higher transfection efficiency and gene silencing than the other studied formulations.",
keywords = "Cationized proteins, Gene therapy, Nanoparticles, pDNA, siRNA",
author = "Zorzi, {Giovanni K.} and P{\'a}rraga, {Jenny E.} and Bego{\~n}a Seijo and Alejandro Sanchez",
year = "2015",
month = "11",
day = "1",
doi = "10.1016/j.colsurfb.2015.08.008",
language = "English",
volume = "135",
pages = "533--541",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Comparison of different cationized proteins as biomaterials for nanoparticle-based ocular gene delivery

AU - Zorzi, Giovanni K.

AU - Párraga, Jenny E.

AU - Seijo, Begoña

AU - Sanchez, Alejandro

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Cationized polymers have been proposed as transfection agents for gene therapy. The present work aims to improve the understanding of the potential use of different cationized proteins (atelocollagen, albumin and gelatin) as nanoparticle components and to investigate the possibility of modulating the physicochemical properties of the resulting nanoparticle carriers by selecting specific protein characteristics in an attempt to improve current ocular gene-delivery approaches. The toxicity profiles, as well as internalization and transfection efficiency, of the developed nanoparticles can be modulated by modifying the molecular weight of the selected protein and the amine used for cationization. The most promising systems are nanoparticles based on intermediate molecular weight gelatin cationized with the endogenous amine spermine, which exhibit an adequate toxicological profile, as well as effective association and protection of pDNA or siRNA molecules, thereby resulting in higher transfection efficiency and gene silencing than the other studied formulations.

AB - Cationized polymers have been proposed as transfection agents for gene therapy. The present work aims to improve the understanding of the potential use of different cationized proteins (atelocollagen, albumin and gelatin) as nanoparticle components and to investigate the possibility of modulating the physicochemical properties of the resulting nanoparticle carriers by selecting specific protein characteristics in an attempt to improve current ocular gene-delivery approaches. The toxicity profiles, as well as internalization and transfection efficiency, of the developed nanoparticles can be modulated by modifying the molecular weight of the selected protein and the amine used for cationization. The most promising systems are nanoparticles based on intermediate molecular weight gelatin cationized with the endogenous amine spermine, which exhibit an adequate toxicological profile, as well as effective association and protection of pDNA or siRNA molecules, thereby resulting in higher transfection efficiency and gene silencing than the other studied formulations.

KW - Cationized proteins

KW - Gene therapy

KW - Nanoparticles

KW - pDNA

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=84939622417&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2015.08.008

DO - 10.1016/j.colsurfb.2015.08.008

M3 - Article

VL - 135

SP - 533

EP - 541

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -