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Computational model for multifocal imaging in optical projection tomography and numerical analysis of all-in-focus fusion in tomographic image reconstruction

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Details

Original languageEnglish
Title of host publicationEMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017
PublisherSpringer Verlag
Pages282-285
Number of pages4
ISBN (Print)9789811051210
DOIs
Publication statusPublished - 2018
Publication typeA4 Article in a conference publication
EventJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC) -
Duration: 1 Jan 1900 → …

Publication series

NameIFMBE Proceedings
Volume65
ISSN (Print)1680-0737

Conference

ConferenceJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC)
Period1/01/00 → …

Abstract

Optical projection tomography (OPT) is a noninvasive 3D imaging method that has been used to study small biological samples. In OPT samples can be mounted in hydrogel scaffold mimicking real life extracellular matrix, and hence grown in all natural dimensions. In optical imaging systems, focusing lenses are required for image acquisition. Due to these lenses, particles at a certain distance from objectives — in the focal plane of the lens — are captured accurately and the further a particle is from the focal plane the blurrier it is captured in the resulting image. To compensate this limitation, multifocal OPT is implemented, where images from each angle are taken with multiple focal planes at different distances. From these images, parts in focus are detected and combined into a single image using all-in-focus fusion algorithm. In this work we present computational way of modeling multifocal imaging and use the presented model to assess the performance of two different all-in-focus fusion methods.

ASJC Scopus subject areas

Keywords

  • All-in-focus fusion, Multifocal microscopy, Optical 3D microscopy, Optical projection tomography, Tomographic image reconstruction

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Field of science, Statistics Finland

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