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Cultivation of Nannochloropsis for eicosapentaenoic acid production in wastewaters of pulp and paper industry

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalBioresource Technology
Early online date2 Jul 2015
Publication statusPublished - 1 Oct 2015
Publication typeA1 Journal article-refereed


The eicosapentaenoic acid (EPA) containing marine microalga Nannochloropsis oculata was grown in an effluent from anaerobic digestion of excess activated sludge from a wastewater treatment plant serving a combination of a pulp and a paper mill and a municipality (digester effluent, DE), mixed with the effluent of the same wastewater treatment plant. The maximum specific growth rate and photosynthesis of N. oculata were similar in the DE medium and in artificial sea water medium (ASW) but after 7. days, algae grown in the DE medium contained seven times more triacylglycerols (TAGs) per cell than cells grown in ASW, indicating mild stress in the DE medium. However, the volumetric rate of EPA production was similar in the ASW and DE media. The results suggest that N. oculata could be used to produce EPA, utilizing the nutrients available after anaerobic digestion of excess activated sludge of a pulp and paper mill.


  • Eicosapentaenoic acid, Nannochloropsis, Paper mill, Pulp mill, Wastewater

Publication forum classification

Field of science, Statistics Finland