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Demonstration of increased lipid accumulation potential of stigeoclonium sp., Kütz. BUM11007 under nitrogen starved regime: A new source of lipids for biodiesel production

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Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalJournal of Biobased Materials and Bioenergy
Issue number2
Publication statusPublished - Apr 2012
Publication typeA1 Journal article-refereed


The fresh water microalga Stigeoclonium sp., Kütz. BUM11007 was investigated for their property to be a suitable candidate for biodiesel production. The growth, lipid content and fatty acid profiles of the organism were determined under both normal and nitrogen free conditions with Chu 10 medium. A maximum biomass concentration 2.84±0.11 g/l with lipid content 138.21±9.82 mg/g and lipid productivity 15.07±0.67 mg/l·d was obtained under nutrient sufficient condition. In contrast to which under nitrogen depleted regimes in a two phase culturing system, biomass yield 2.798±0.18 g/l with increased lipid content 407.18 ± 11.6 mg/g at lipid productivity 43.68 ± 1.82 mg/l · d were recorded. The fatty acid methyl ester profiles revealed the presence of 16:0 (palmitic), 18:0 (stearic), 18:1 (oleic) and 18:2 (linoleic) methyl esters as the major components. The results show the ability of the algae to be a promising feedstock source for biodiesel production.


  • Biodiesel, FAME Production, Lipid Extraction, Nitrogen Starvation, Stigeoclonium