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Detection and Segmentation of Nucleoids Based on Gradient Path Labelling

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Detection and Segmentation of Nucleoids Based on Gradient Path Labelling. / Santinha, Joao; Mora, Andre D.; Fonseca, Jose; Goncalves, Nadia; Ribeiro, Andre S.

In: International Journal of Pharma Medicine and Biological Sciences, Vol. 4, No. 1, 2015, p. 51-55.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Santinha, J, Mora, AD, Fonseca, J, Goncalves, N & Ribeiro, AS 2015, 'Detection and Segmentation of Nucleoids Based on Gradient Path Labelling', International Journal of Pharma Medicine and Biological Sciences, vol. 4, no. 1, pp. 51-55.

APA

Santinha, J., Mora, A. D., Fonseca, J., Goncalves, N., & Ribeiro, A. S. (2015). Detection and Segmentation of Nucleoids Based on Gradient Path Labelling. International Journal of Pharma Medicine and Biological Sciences, 4(1), 51-55.

Vancouver

Santinha J, Mora AD, Fonseca J, Goncalves N, Ribeiro AS. Detection and Segmentation of Nucleoids Based on Gradient Path Labelling. International Journal of Pharma Medicine and Biological Sciences. 2015;4(1):51-55.

Author

Santinha, Joao ; Mora, Andre D. ; Fonseca, Jose ; Goncalves, Nadia ; Ribeiro, Andre S. / Detection and Segmentation of Nucleoids Based on Gradient Path Labelling. In: International Journal of Pharma Medicine and Biological Sciences. 2015 ; Vol. 4, No. 1. pp. 51-55.

Bibtex - Download

@article{ac7767c3da4d4ccabfd35d5521ddb029,
title = "Detection and Segmentation of Nucleoids Based on Gradient Path Labelling",
abstract = "Cellular aging is one of the topics that live cell imaging can assist. With age, there is an increase of aggregates of misfolded proteins, to which age-related diseases have been linked to. In Escherichia coli, protein aggregates linked to its aging process exhibit a spatial distribution that appears to be caused by the nucleoid at midcell. To correlate the locations of protein aggregates and the nucleoid, it is necessary to detect and segment the nucleoid from microscopy images. We present an adaptation of methods for Drusens’ detection and segmentation to nucleoids in E. coli. The size of the nucleoid, extracted using the method here proposed, was compared with an alternative measure (FWHM-based measure) and with the regions of anisotropies in aggregates motions. These comparisons suggest that our new method is of use, providing more accurate minor axis lengths. Also, it provides additional measures, such as the nucleoid’s center orientation angle, area, and pixel list.",
author = "Joao Santinha and Mora, {Andre D.} and Jose Fonseca and Nadia Goncalves and Ribeiro, {Andre S.}",
year = "2015",
language = "English",
volume = "4",
pages = "51--55",
journal = "International Journal of Pharma Medicine and Biological Sciences",
issn = "2278-5221",
publisher = "International Journal of Pharma Medicine and Biological Sciences",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Detection and Segmentation of Nucleoids Based on Gradient Path Labelling

AU - Santinha, Joao

AU - Mora, Andre D.

AU - Fonseca, Jose

AU - Goncalves, Nadia

AU - Ribeiro, Andre S.

PY - 2015

Y1 - 2015

N2 - Cellular aging is one of the topics that live cell imaging can assist. With age, there is an increase of aggregates of misfolded proteins, to which age-related diseases have been linked to. In Escherichia coli, protein aggregates linked to its aging process exhibit a spatial distribution that appears to be caused by the nucleoid at midcell. To correlate the locations of protein aggregates and the nucleoid, it is necessary to detect and segment the nucleoid from microscopy images. We present an adaptation of methods for Drusens’ detection and segmentation to nucleoids in E. coli. The size of the nucleoid, extracted using the method here proposed, was compared with an alternative measure (FWHM-based measure) and with the regions of anisotropies in aggregates motions. These comparisons suggest that our new method is of use, providing more accurate minor axis lengths. Also, it provides additional measures, such as the nucleoid’s center orientation angle, area, and pixel list.

AB - Cellular aging is one of the topics that live cell imaging can assist. With age, there is an increase of aggregates of misfolded proteins, to which age-related diseases have been linked to. In Escherichia coli, protein aggregates linked to its aging process exhibit a spatial distribution that appears to be caused by the nucleoid at midcell. To correlate the locations of protein aggregates and the nucleoid, it is necessary to detect and segment the nucleoid from microscopy images. We present an adaptation of methods for Drusens’ detection and segmentation to nucleoids in E. coli. The size of the nucleoid, extracted using the method here proposed, was compared with an alternative measure (FWHM-based measure) and with the regions of anisotropies in aggregates motions. These comparisons suggest that our new method is of use, providing more accurate minor axis lengths. Also, it provides additional measures, such as the nucleoid’s center orientation angle, area, and pixel list.

M3 - Article

VL - 4

SP - 51

EP - 55

JO - International Journal of Pharma Medicine and Biological Sciences

JF - International Journal of Pharma Medicine and Biological Sciences

SN - 2278-5221

IS - 1

ER -