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Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators

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Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators. / Auer, Sanna; Nirschl, Martin; Schreiter, Matthias; Vikholm-Lundin, Inger.

In: Analytical and Bioanalytical Chemistry, Vol. 400, No. 5, 05.2011, p. 1387-1396.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Auer, S, Nirschl, M, Schreiter, M & Vikholm-Lundin, I 2011, 'Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators', Analytical and Bioanalytical Chemistry, vol. 400, no. 5, pp. 1387-1396. https://doi.org/10.1007/s00216-011-4871-0

APA

Auer, S., Nirschl, M., Schreiter, M., & Vikholm-Lundin, I. (2011). Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators. Analytical and Bioanalytical Chemistry, 400(5), 1387-1396. https://doi.org/10.1007/s00216-011-4871-0

Vancouver

Auer S, Nirschl M, Schreiter M, Vikholm-Lundin I. Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators. Analytical and Bioanalytical Chemistry. 2011 May;400(5):1387-1396. https://doi.org/10.1007/s00216-011-4871-0

Author

Auer, Sanna ; Nirschl, Martin ; Schreiter, Matthias ; Vikholm-Lundin, Inger. / Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 400, No. 5. pp. 1387-1396.

Bibtex - Download

@article{5d7974f4a1234bd592d12d04c92d1111,
title = "Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators",
abstract = "Nanomolar quantities of single-stranded DNA products ∼ 100 nucleotides long can be detected in diluted 1{\%} serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the acoustic and the optical device. Oligonucleotides and a repellent lipoamide, Lipa-DEA, were allowed to assemble on the sensor chip surfaces for only 15 min by dispensing. Lipa-DEA surrounds the analyte-binding probes on the surface and effectively reduces the non-specific binding of bovine serum albumin and non-complementary strands. In a highly diluted serum matrix, the non-specific binding is, however, a hindrance, and the background response must be reduced. Nanomolar concentrations of short complementary oligos could be detected in buffer, whereas the response was too low to be measured in serum. DNA strands that are approximately 100 base pairs long at concentrations as low as 1-nM could be detected both in buffer and in 1{\%} serum by both SPR and the FBAR resonator.",
keywords = "DNA hybridisation detection, DNA sensor, Film bulk acoustic resonator, Self-assembled monolayer, Serum, Surface plasmon resonance",
author = "Sanna Auer and Martin Nirschl and Matthias Schreiter and Inger Vikholm-Lundin",
year = "2011",
month = "5",
doi = "10.1007/s00216-011-4871-0",
language = "English",
volume = "400",
pages = "1387--1396",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators

AU - Auer, Sanna

AU - Nirschl, Martin

AU - Schreiter, Matthias

AU - Vikholm-Lundin, Inger

PY - 2011/5

Y1 - 2011/5

N2 - Nanomolar quantities of single-stranded DNA products ∼ 100 nucleotides long can be detected in diluted 1% serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the acoustic and the optical device. Oligonucleotides and a repellent lipoamide, Lipa-DEA, were allowed to assemble on the sensor chip surfaces for only 15 min by dispensing. Lipa-DEA surrounds the analyte-binding probes on the surface and effectively reduces the non-specific binding of bovine serum albumin and non-complementary strands. In a highly diluted serum matrix, the non-specific binding is, however, a hindrance, and the background response must be reduced. Nanomolar concentrations of short complementary oligos could be detected in buffer, whereas the response was too low to be measured in serum. DNA strands that are approximately 100 base pairs long at concentrations as low as 1-nM could be detected both in buffer and in 1% serum by both SPR and the FBAR resonator.

AB - Nanomolar quantities of single-stranded DNA products ∼ 100 nucleotides long can be detected in diluted 1% serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the acoustic and the optical device. Oligonucleotides and a repellent lipoamide, Lipa-DEA, were allowed to assemble on the sensor chip surfaces for only 15 min by dispensing. Lipa-DEA surrounds the analyte-binding probes on the surface and effectively reduces the non-specific binding of bovine serum albumin and non-complementary strands. In a highly diluted serum matrix, the non-specific binding is, however, a hindrance, and the background response must be reduced. Nanomolar concentrations of short complementary oligos could be detected in buffer, whereas the response was too low to be measured in serum. DNA strands that are approximately 100 base pairs long at concentrations as low as 1-nM could be detected both in buffer and in 1% serum by both SPR and the FBAR resonator.

KW - DNA hybridisation detection

KW - DNA sensor

KW - Film bulk acoustic resonator

KW - Self-assembled monolayer

KW - Serum

KW - Surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=79955562374&partnerID=8YFLogxK

U2 - 10.1007/s00216-011-4871-0

DO - 10.1007/s00216-011-4871-0

M3 - Article

VL - 400

SP - 1387

EP - 1396

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 5

ER -