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Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells

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Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells. / Ahtiainen, Katja; Sippola, Laura; Nurminen, Manu; Mannerström, Bettina; Haimi, Suvi; Suuronen, Riitta; Hyttinen, Jari; Ylikomi, Timo; Kellomäki, Minna; Miettinen, Susanna.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, No. 1, 01.01.2015, p. 55-65.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Ahtiainen, K, Sippola, L, Nurminen, M, Mannerström, B, Haimi, S, Suuronen, R, Hyttinen, J, Ylikomi, T, Kellomäki, M & Miettinen, S 2015, 'Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells', Journal of Tissue Engineering and Regenerative Medicine, vol. 9, no. 1, pp. 55-65. https://doi.org/10.1002/term.1614

APA

Ahtiainen, K., Sippola, L., Nurminen, M., Mannerström, B., Haimi, S., Suuronen, R., ... Miettinen, S. (2015). Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(1), 55-65. https://doi.org/10.1002/term.1614

Vancouver

Ahtiainen K, Sippola L, Nurminen M, Mannerström B, Haimi S, Suuronen R et al. Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells. Journal of Tissue Engineering and Regenerative Medicine. 2015 Jan 1;9(1):55-65. https://doi.org/10.1002/term.1614

Author

Ahtiainen, Katja ; Sippola, Laura ; Nurminen, Manu ; Mannerström, Bettina ; Haimi, Suvi ; Suuronen, Riitta ; Hyttinen, Jari ; Ylikomi, Timo ; Kellomäki, Minna ; Miettinen, Susanna. / Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2015 ; Vol. 9, No. 1. pp. 55-65.

Bibtex - Download

@article{5221f17bd04442399e549735fa38778f,
title = "Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells",
abstract = "The performance of biodegradable knitted and rolled 3-dimensional (3D) polylactide-based 96/4 scaffolds modified with bioactive glass (BaG) 13-93, chitosan and both was compared with regard to the viability, proliferation and chondrogenic differentiation of rabbit adipose stem cells (ASCs). Scaffold porosities were determined by micro-computed tomography (μCT). Water absorption and degradation of scaffolds were studied during 28-day hydrolysis in Tris-buffer. Viability, number and differentiation of ASCs in PLA96/4 scaffolds were examined in vitro. The dimensions of the scaffolds were maintained during hydrolysis and mass loss was detected only in the BaG13-93 containing scaffolds. ASCs adhered and proliferated on each scaffold type. Cell aggregation and expression of chondral matrix components improved in all scaffold types in chondrogenic medium. Signs of hypertrophy were detected in the modified scaffolds but not in the plain PLA96/4 scaffold. Chondrogenic differentiation was most enhanced in the presence of chitosan. These findings indicate that the plain P scaffold provided a good 3D-matrix for ASC proliferation whereas the addition of chitosan to the PLA96/4 scaffold induced chondrogenic differentiation independent of the medium. Accordingly, a PLA96/4 scaffold modified by chitosan could provide a functional and bioactive basis for tissue-engineered chondral implants.",
keywords = "Adipose stem cell, Bioactive glass, Chitosan, Chondrogenesis, Polylactide, Scaffold, Tissue engineering",
author = "Katja Ahtiainen and Laura Sippola and Manu Nurminen and Bettina Mannerstr{\"o}m and Suvi Haimi and Riitta Suuronen and Jari Hyttinen and Timo Ylikomi and Minna Kellom{\"a}ki and Susanna Miettinen",
note = "Early view : Article first published online: 22 OCT 2012 : J Tissue Eng Regn Med.(2012) DOI: 10.1002/term.1614 : Ei viel{\"a} WoS 2012-12-28 : UT puuttuu : Poistettu tupla r=4051. ei ut-numeroa 9.8.2013<br/>Contribution: organisation=bme,FACT1=1<br/>Publisher name: John Wiley & Sons, Inc.",
year = "2015",
month = "1",
day = "1",
doi = "10.1002/term.1614",
language = "English",
volume = "9",
pages = "55--65",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "Wiley",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Effects of chitosan and bioactive glass modifications of knitted and rolled polylactide-based 96/4L/D scaffolds on chondrogenic differentiation of adipose stem cells

AU - Ahtiainen, Katja

AU - Sippola, Laura

AU - Nurminen, Manu

AU - Mannerström, Bettina

AU - Haimi, Suvi

AU - Suuronen, Riitta

AU - Hyttinen, Jari

AU - Ylikomi, Timo

AU - Kellomäki, Minna

AU - Miettinen, Susanna

N1 - Early view : Article first published online: 22 OCT 2012 : J Tissue Eng Regn Med.(2012) DOI: 10.1002/term.1614 : Ei vielä WoS 2012-12-28 : UT puuttuu : Poistettu tupla r=4051. ei ut-numeroa 9.8.2013<br/>Contribution: organisation=bme,FACT1=1<br/>Publisher name: John Wiley & Sons, Inc.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The performance of biodegradable knitted and rolled 3-dimensional (3D) polylactide-based 96/4 scaffolds modified with bioactive glass (BaG) 13-93, chitosan and both was compared with regard to the viability, proliferation and chondrogenic differentiation of rabbit adipose stem cells (ASCs). Scaffold porosities were determined by micro-computed tomography (μCT). Water absorption and degradation of scaffolds were studied during 28-day hydrolysis in Tris-buffer. Viability, number and differentiation of ASCs in PLA96/4 scaffolds were examined in vitro. The dimensions of the scaffolds were maintained during hydrolysis and mass loss was detected only in the BaG13-93 containing scaffolds. ASCs adhered and proliferated on each scaffold type. Cell aggregation and expression of chondral matrix components improved in all scaffold types in chondrogenic medium. Signs of hypertrophy were detected in the modified scaffolds but not in the plain PLA96/4 scaffold. Chondrogenic differentiation was most enhanced in the presence of chitosan. These findings indicate that the plain P scaffold provided a good 3D-matrix for ASC proliferation whereas the addition of chitosan to the PLA96/4 scaffold induced chondrogenic differentiation independent of the medium. Accordingly, a PLA96/4 scaffold modified by chitosan could provide a functional and bioactive basis for tissue-engineered chondral implants.

AB - The performance of biodegradable knitted and rolled 3-dimensional (3D) polylactide-based 96/4 scaffolds modified with bioactive glass (BaG) 13-93, chitosan and both was compared with regard to the viability, proliferation and chondrogenic differentiation of rabbit adipose stem cells (ASCs). Scaffold porosities were determined by micro-computed tomography (μCT). Water absorption and degradation of scaffolds were studied during 28-day hydrolysis in Tris-buffer. Viability, number and differentiation of ASCs in PLA96/4 scaffolds were examined in vitro. The dimensions of the scaffolds were maintained during hydrolysis and mass loss was detected only in the BaG13-93 containing scaffolds. ASCs adhered and proliferated on each scaffold type. Cell aggregation and expression of chondral matrix components improved in all scaffold types in chondrogenic medium. Signs of hypertrophy were detected in the modified scaffolds but not in the plain PLA96/4 scaffold. Chondrogenic differentiation was most enhanced in the presence of chitosan. These findings indicate that the plain P scaffold provided a good 3D-matrix for ASC proliferation whereas the addition of chitosan to the PLA96/4 scaffold induced chondrogenic differentiation independent of the medium. Accordingly, a PLA96/4 scaffold modified by chitosan could provide a functional and bioactive basis for tissue-engineered chondral implants.

KW - Adipose stem cell

KW - Bioactive glass

KW - Chitosan

KW - Chondrogenesis

KW - Polylactide

KW - Scaffold

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84920569684&partnerID=8YFLogxK

U2 - 10.1002/term.1614

DO - 10.1002/term.1614

M3 - Article

VL - 9

SP - 55

EP - 65

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 1

ER -