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Effects of exogenous alpha-synuclein on stimulated dopamine overflow in dorsal striatum

Research output: Contribution to journalArticleScientificpeer-review

Details

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalNeuroscience Letters
Volume554
DOIs
Publication statusPublished - 25 Oct 2013
Publication typeA1 Journal article-refereed

Abstract

Alpha-synuclein (α-syn) is mainly a presynaptic protein that has been implicated in Parkinson's disease and various other neurodegenerative disorders. Evidence obtained in knockout mice suggests that α-syn controls plasticity of dopamine (DA) overflow in presynaptic terminals. It is also believed that α-syn spreads and may seed its aggregates from cell to cell. The effects of exogenously applied α-syn on dopaminergic neurotransmission have not been studied. We addressed this issue by microinjecting human α-syn protein into the dorsal striatum of wild-type and α-syn knockout mice and monitoring stimulated DA overflow with constant potential amperometry. The evoked DA overflow was decreased in knockout mice six days after α-syn microinjection. The maximal velocity of DA re-uptake was reduced in both genotypes. Similar results were not seen when the effects of microinjected α-syn were studied immediately after the treatment, but instead there was a trend toward an increase in both stimulated DA overflow and maximal velocity of DA re-uptake. We conclude that locally applied human α-syn affects DA overflow and the effects depend on the presence of endogenous α-syn.

ASJC Scopus subject areas

Keywords

  • Alpha-synuclein, Constant potential amperometry, Dopamine overflow, Knockout mice, Microinjection