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Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

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Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying. / Auvinen, Vili-Veli; Merivaara, Arto; Kiiskinen, Jasmi; Paukkonen, Heli; Laurén, Patrick; Hakkarainen, Tiina; Koivuniemi, Raili; Sarkanen, Riina; Ylikomi, Timo; Laaksonen, Timo; Yliperttula, Marjo.

In: Cryobiology, 15.09.2019.

Research output: Contribution to journalArticleScientificpeer-review

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Auvinen, V-V, Merivaara, A, Kiiskinen, J, Paukkonen, H, Laurén, P, Hakkarainen, T, Koivuniemi, R, Sarkanen, R, Ylikomi, T, Laaksonen, T & Yliperttula, M 2019, 'Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying', Cryobiology. https://doi.org/10.1016/j.cryobiol.2019.09.005

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Auvinen, Vili-Veli ; Merivaara, Arto ; Kiiskinen, Jasmi ; Paukkonen, Heli ; Laurén, Patrick ; Hakkarainen, Tiina ; Koivuniemi, Raili ; Sarkanen, Riina ; Ylikomi, Timo ; Laaksonen, Timo ; Yliperttula, Marjo. / Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying. In: Cryobiology. 2019.

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@article{58ea583a67df446e8867e1d7f33b9a4d,
title = "Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying",
abstract = "The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18{\%} of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals.",
keywords = "3D cell culture, Adipose tissue extract, Cell spheroids, Freeze-drying, Nanofibrillated cellulose",
author = "Vili-Veli Auvinen and Arto Merivaara and Jasmi Kiiskinen and Heli Paukkonen and Patrick Laur{\'e}n and Tiina Hakkarainen and Raili Koivuniemi and Riina Sarkanen and Timo Ylikomi and Timo Laaksonen and Marjo Yliperttula",
note = "dupl=50229030",
year = "2019",
month = "9",
day = "15",
doi = "10.1016/j.cryobiol.2019.09.005",
language = "English",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

AU - Auvinen, Vili-Veli

AU - Merivaara, Arto

AU - Kiiskinen, Jasmi

AU - Paukkonen, Heli

AU - Laurén, Patrick

AU - Hakkarainen, Tiina

AU - Koivuniemi, Raili

AU - Sarkanen, Riina

AU - Ylikomi, Timo

AU - Laaksonen, Timo

AU - Yliperttula, Marjo

N1 - dupl=50229030

PY - 2019/9/15

Y1 - 2019/9/15

N2 - The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18% of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals.

AB - The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18% of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals.

KW - 3D cell culture

KW - Adipose tissue extract

KW - Cell spheroids

KW - Freeze-drying

KW - Nanofibrillated cellulose

U2 - 10.1016/j.cryobiol.2019.09.005

DO - 10.1016/j.cryobiol.2019.09.005

M3 - Article

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

ER -