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Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering

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Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. / Björninen, Miina; Gilmore, Kerry; Pelto, Jani; Seppänen-Kaijansinkko, Riitta; Kellomäki, Minna; Miettinen, Susanna; Wallace, Gordon; Grijpma, Dirk; Haimi, Suvi.

In: Annals of Biomedical Engineering, 14.11.2016, p. 1-12.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Björninen, M, Gilmore, K, Pelto, J, Seppänen-Kaijansinkko, R, Kellomäki, M, Miettinen, S, Wallace, G, Grijpma, D & Haimi, S 2016, 'Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering', Annals of Biomedical Engineering, pp. 1-12. https://doi.org/10.1007/s10439-016-1755-7

APA

Björninen, M., Gilmore, K., Pelto, J., Seppänen-Kaijansinkko, R., Kellomäki, M., Miettinen, S., ... Haimi, S. (2016). Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. Annals of Biomedical Engineering, 1-12. https://doi.org/10.1007/s10439-016-1755-7

Vancouver

Björninen M, Gilmore K, Pelto J, Seppänen-Kaijansinkko R, Kellomäki M, Miettinen S et al. Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. Annals of Biomedical Engineering. 2016 Nov 14;1-12. https://doi.org/10.1007/s10439-016-1755-7

Author

Björninen, Miina ; Gilmore, Kerry ; Pelto, Jani ; Seppänen-Kaijansinkko, Riitta ; Kellomäki, Minna ; Miettinen, Susanna ; Wallace, Gordon ; Grijpma, Dirk ; Haimi, Suvi. / Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. In: Annals of Biomedical Engineering. 2016 ; pp. 1-12.

Bibtex - Download

@article{4530623fd455468682f7b429ec23308d,
title = "Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering",
abstract = "We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.",
author = "Miina Bj{\"o}rninen and Kerry Gilmore and Jani Pelto and Riitta Sepp{\"a}nen-Kaijansinkko and Minna Kellom{\"a}ki and Susanna Miettinen and Gordon Wallace and Dirk Grijpma and Suvi Haimi",
note = "EXT={"}Pelto, Jani{"}",
year = "2016",
month = "11",
day = "14",
doi = "10.1007/s10439-016-1755-7",
language = "English",
pages = "1--12",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
publisher = "Springer Verlag",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering

AU - Björninen, Miina

AU - Gilmore, Kerry

AU - Pelto, Jani

AU - Seppänen-Kaijansinkko, Riitta

AU - Kellomäki, Minna

AU - Miettinen, Susanna

AU - Wallace, Gordon

AU - Grijpma, Dirk

AU - Haimi, Suvi

N1 - EXT="Pelto, Jani"

PY - 2016/11/14

Y1 - 2016/11/14

N2 - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.

AB - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.

U2 - 10.1007/s10439-016-1755-7

DO - 10.1007/s10439-016-1755-7

M3 - Article

SP - 1

EP - 12

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

SN - 0090-6964

ER -