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Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material

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Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material. / Mangayil, Rahul; Rajala, Satu; Pammo, Arno; Sarlin, Essi; Luo, Jin; Santala, Ville; Karp, Matti; Tuukkanen, Sampo.

In: ACS Applied Materials & Interfaces, Vol. 9, No. 22, 2017, p. 19048–19056.

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@article{9b730b8dc29244a19013d33a61067a86,
title = "Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material",
abstract = "Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity, and superior mechanical and structural properties. Aiming toward improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production. The changes in cell growth, end metabolite, and BC production titers from the engineered strains were compared with the wild-type K. xylinus. Although there were no significant differences between the growth of wild-type and engineered strains, the engineered K. xylinus strains demonstrated faster BC production, generating 2–4-fold higher production titer (the highest observed titer was obtained with K. xylinus-bcsABCD strain producing 4.3 ± 0.46 g/L BC in 4 days). The mechanical and structural characteristics of cellulose produced from the wild-type and engineered K. xylinus strains were analyzed with a stylus profilometer, in-house built tensile strength measurement system, a scanning electron microscope, and an X-ray diffractometer. Results from the profilometer indicated that the engineered K. xylinus strains produced thicker BC films (wild type, 5.1 μm, and engineered K. xylinus strains, 6.2–10.2 μm). Scanning electron microscope revealed no principal differences in the structure of the different type BC films. The crystallinity index of all films was high (from 88.6 to 97.5{\%}). All BC films showed significant piezoelectric response (5.0–20 pC/N), indicating BC as a promising sensor material.",
author = "Rahul Mangayil and Satu Rajala and Arno Pammo and Essi Sarlin and Jin Luo and Ville Santala and Matti Karp and Sampo Tuukkanen",
year = "2017",
doi = "10.1021/acsami.7b04927",
language = "English",
volume = "9",
pages = "19048–19056",
journal = "ACS Applied Materials & Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society ACS",
number = "22",

}

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TY - JOUR

T1 - Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material

AU - Mangayil, Rahul

AU - Rajala, Satu

AU - Pammo, Arno

AU - Sarlin, Essi

AU - Luo, Jin

AU - Santala, Ville

AU - Karp, Matti

AU - Tuukkanen, Sampo

PY - 2017

Y1 - 2017

N2 - Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity, and superior mechanical and structural properties. Aiming toward improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production. The changes in cell growth, end metabolite, and BC production titers from the engineered strains were compared with the wild-type K. xylinus. Although there were no significant differences between the growth of wild-type and engineered strains, the engineered K. xylinus strains demonstrated faster BC production, generating 2–4-fold higher production titer (the highest observed titer was obtained with K. xylinus-bcsABCD strain producing 4.3 ± 0.46 g/L BC in 4 days). The mechanical and structural characteristics of cellulose produced from the wild-type and engineered K. xylinus strains were analyzed with a stylus profilometer, in-house built tensile strength measurement system, a scanning electron microscope, and an X-ray diffractometer. Results from the profilometer indicated that the engineered K. xylinus strains produced thicker BC films (wild type, 5.1 μm, and engineered K. xylinus strains, 6.2–10.2 μm). Scanning electron microscope revealed no principal differences in the structure of the different type BC films. The crystallinity index of all films was high (from 88.6 to 97.5%). All BC films showed significant piezoelectric response (5.0–20 pC/N), indicating BC as a promising sensor material.

AB - Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity, and superior mechanical and structural properties. Aiming toward improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production. The changes in cell growth, end metabolite, and BC production titers from the engineered strains were compared with the wild-type K. xylinus. Although there were no significant differences between the growth of wild-type and engineered strains, the engineered K. xylinus strains demonstrated faster BC production, generating 2–4-fold higher production titer (the highest observed titer was obtained with K. xylinus-bcsABCD strain producing 4.3 ± 0.46 g/L BC in 4 days). The mechanical and structural characteristics of cellulose produced from the wild-type and engineered K. xylinus strains were analyzed with a stylus profilometer, in-house built tensile strength measurement system, a scanning electron microscope, and an X-ray diffractometer. Results from the profilometer indicated that the engineered K. xylinus strains produced thicker BC films (wild type, 5.1 μm, and engineered K. xylinus strains, 6.2–10.2 μm). Scanning electron microscope revealed no principal differences in the structure of the different type BC films. The crystallinity index of all films was high (from 88.6 to 97.5%). All BC films showed significant piezoelectric response (5.0–20 pC/N), indicating BC as a promising sensor material.

U2 - 10.1021/acsami.7b04927

DO - 10.1021/acsami.7b04927

M3 - Article

VL - 9

SP - 19048

EP - 19056

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

SN - 1944-8244

IS - 22

ER -