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Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery

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Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery. / Nikkola, Lila; Morton, Tatjana; Balmayor, Elizabeth R.; Jukola, Hanna; Harlin, Ali; Redl, Heinz; Van Griensven, Martijn; Ashammakhi, Nureddin.

In: European Journal of Medical Research, Vol. 20, No. 1, 54, 05.06.2015.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Nikkola, L, Morton, T, Balmayor, ER, Jukola, H, Harlin, A, Redl, H, Van Griensven, M & Ashammakhi, N 2015, 'Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery', European Journal of Medical Research, vol. 20, no. 1, 54. https://doi.org/10.1186/s40001-015-0145-1

APA

Nikkola, L., Morton, T., Balmayor, E. R., Jukola, H., Harlin, A., Redl, H., ... Ashammakhi, N. (2015). Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery. European Journal of Medical Research, 20(1), [54]. https://doi.org/10.1186/s40001-015-0145-1

Vancouver

Nikkola L, Morton T, Balmayor ER, Jukola H, Harlin A, Redl H et al. Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery. European Journal of Medical Research. 2015 Jun 5;20(1). 54. https://doi.org/10.1186/s40001-015-0145-1

Author

Nikkola, Lila ; Morton, Tatjana ; Balmayor, Elizabeth R. ; Jukola, Hanna ; Harlin, Ali ; Redl, Heinz ; Van Griensven, Martijn ; Ashammakhi, Nureddin. / Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery. In: European Journal of Medical Research. 2015 ; Vol. 20, No. 1.

Bibtex - Download

@article{ae480b41cae042e3a5b2f2b991dff733,
title = "Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery",
abstract = "Background: Adaptation of nanotechnology into materials science has also advanced tissue engineering research. Tissues are basically composed of nanoscale structures hence making nanofibrous materials closely resemble natural fibers. Adding a drug release function to such material may further advance their use in tissue repair. Methods: In the current study, bioabsorbable poly(D,L lactide-co-glycolide)80/20 (PDLGA80/20) was dissolved in a mixture of acetone/dimethylformamide. Twenty percent of diclofenac sodium was added to the solution. Nanofibers were manufactured using electrospinning. The morphology of the obtained scaffolds was analyzed by scanning electron microscopy (SEM). The release of the diclofenac sodium was assessed by UV/Vis spectroscopy. Mouse fibroblasts (MC3T3) were seeded on the scaffolds, and the cell attachment was evaluated with fluorescent microscopy. Results: The thickness of electrospun nanomats was about 1 mm. SEM analysis showed that polymeric nanofibers containing drug particles formed very interconnected porous nanostructures. The average diameter of the nanofibers was 500 nm. Drug release was measured by means of UV/Vis spectroscopy. After a high start peak, the release rate decreased considerably during 11 days and lasted about 60 days. During the evaluation of the release kinetics, a material degradation process was observed. MC3T3 cells attached to the diclofenac sodium-loaded scaffold. Conclusions: The nanofibrous porous structure made of PDLGA polymer loaded with diclofenac sodium is feasible to develop, and it may help to improve biomaterial properties for controlled tissue repair and regeneration.",
keywords = "Biodegradable, Diclofenac sodium, Drug release, Electrospinning, Poly(D,L lactide-co-glycolide), Scaffold",
author = "Lila Nikkola and Tatjana Morton and Balmayor, {Elizabeth R.} and Hanna Jukola and Ali Harlin and Heinz Redl and {Van Griensven}, Martijn and Nureddin Ashammakhi",
note = "ORG=elt,0.5 ORG=mol,0.5",
year = "2015",
month = "6",
day = "5",
doi = "10.1186/s40001-015-0145-1",
language = "English",
volume = "20",
journal = "European Journal of Medical Research",
issn = "0949-2321",
publisher = "BioMed Central",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Fabrication of electrospun poly(D,L lactide-co-glycolide)80/20 scaffolds loaded with diclofenac sodium for tissue engineering Surgery

AU - Nikkola, Lila

AU - Morton, Tatjana

AU - Balmayor, Elizabeth R.

AU - Jukola, Hanna

AU - Harlin, Ali

AU - Redl, Heinz

AU - Van Griensven, Martijn

AU - Ashammakhi, Nureddin

N1 - ORG=elt,0.5 ORG=mol,0.5

PY - 2015/6/5

Y1 - 2015/6/5

N2 - Background: Adaptation of nanotechnology into materials science has also advanced tissue engineering research. Tissues are basically composed of nanoscale structures hence making nanofibrous materials closely resemble natural fibers. Adding a drug release function to such material may further advance their use in tissue repair. Methods: In the current study, bioabsorbable poly(D,L lactide-co-glycolide)80/20 (PDLGA80/20) was dissolved in a mixture of acetone/dimethylformamide. Twenty percent of diclofenac sodium was added to the solution. Nanofibers were manufactured using electrospinning. The morphology of the obtained scaffolds was analyzed by scanning electron microscopy (SEM). The release of the diclofenac sodium was assessed by UV/Vis spectroscopy. Mouse fibroblasts (MC3T3) were seeded on the scaffolds, and the cell attachment was evaluated with fluorescent microscopy. Results: The thickness of electrospun nanomats was about 1 mm. SEM analysis showed that polymeric nanofibers containing drug particles formed very interconnected porous nanostructures. The average diameter of the nanofibers was 500 nm. Drug release was measured by means of UV/Vis spectroscopy. After a high start peak, the release rate decreased considerably during 11 days and lasted about 60 days. During the evaluation of the release kinetics, a material degradation process was observed. MC3T3 cells attached to the diclofenac sodium-loaded scaffold. Conclusions: The nanofibrous porous structure made of PDLGA polymer loaded with diclofenac sodium is feasible to develop, and it may help to improve biomaterial properties for controlled tissue repair and regeneration.

AB - Background: Adaptation of nanotechnology into materials science has also advanced tissue engineering research. Tissues are basically composed of nanoscale structures hence making nanofibrous materials closely resemble natural fibers. Adding a drug release function to such material may further advance their use in tissue repair. Methods: In the current study, bioabsorbable poly(D,L lactide-co-glycolide)80/20 (PDLGA80/20) was dissolved in a mixture of acetone/dimethylformamide. Twenty percent of diclofenac sodium was added to the solution. Nanofibers were manufactured using electrospinning. The morphology of the obtained scaffolds was analyzed by scanning electron microscopy (SEM). The release of the diclofenac sodium was assessed by UV/Vis spectroscopy. Mouse fibroblasts (MC3T3) were seeded on the scaffolds, and the cell attachment was evaluated with fluorescent microscopy. Results: The thickness of electrospun nanomats was about 1 mm. SEM analysis showed that polymeric nanofibers containing drug particles formed very interconnected porous nanostructures. The average diameter of the nanofibers was 500 nm. Drug release was measured by means of UV/Vis spectroscopy. After a high start peak, the release rate decreased considerably during 11 days and lasted about 60 days. During the evaluation of the release kinetics, a material degradation process was observed. MC3T3 cells attached to the diclofenac sodium-loaded scaffold. Conclusions: The nanofibrous porous structure made of PDLGA polymer loaded with diclofenac sodium is feasible to develop, and it may help to improve biomaterial properties for controlled tissue repair and regeneration.

KW - Biodegradable

KW - Diclofenac sodium

KW - Drug release

KW - Electrospinning

KW - Poly(D,L lactide-co-glycolide)

KW - Scaffold

UR - http://www.scopus.com/inward/record.url?scp=84931269207&partnerID=8YFLogxK

U2 - 10.1186/s40001-015-0145-1

DO - 10.1186/s40001-015-0145-1

M3 - Article

VL - 20

JO - European Journal of Medical Research

JF - European Journal of Medical Research

SN - 0949-2321

IS - 1

M1 - 54

ER -