Tampere University of Technology

TUTCRIS Research Portal

Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state

Research output: Contribution to journalArticleScientificpeer-review

Standard

Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state. / Meng, Lu; Alter, Thomas; Aho, Tommi; Huehn, Stephan.

In: FEMS Microbiology Ecology, Vol. 91, No. 5, 035, 05.2015.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Meng, L, Alter, T, Aho, T & Huehn, S 2015, 'Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state', FEMS Microbiology Ecology, vol. 91, no. 5, 035. https://doi.org/10.1093/femsec/fiv035

APA

Meng, L., Alter, T., Aho, T., & Huehn, S. (2015). Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state. FEMS Microbiology Ecology, 91(5), [035]. https://doi.org/10.1093/femsec/fiv035

Vancouver

Meng L, Alter T, Aho T, Huehn S. Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state. FEMS Microbiology Ecology. 2015 May;91(5). 035. https://doi.org/10.1093/femsec/fiv035

Author

Meng, Lu ; Alter, Thomas ; Aho, Tommi ; Huehn, Stephan. / Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state. In: FEMS Microbiology Ecology. 2015 ; Vol. 91, No. 5.

Bibtex - Download

@article{e4144b33043148168907e944a4748415,
title = "Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state",
abstract = "Viable but non-culturable (VBNC) state is referred to as a dormant state of non-sporulating bacteria enhancing the survival in adverse environments. To our knowledge, only few studies have been conducted on whole genomic expression of Vibrio parahaemolyticus VBNC state. Since a degradation of nucleic acids in V. vulnificus non-culturable state has been detected, we hypothesize that gene regulation of VBNC cells is highly reduced, downregulation of gene expression is dominant and only metabolic functions crucial for survival are kept on a sustained basis. Hence, we performed the whole transcriptomic profiles of V. parahaemolyticus in three phases (exponential, early stationary phase and VBNC state). Compared with exponential and early stationary phase, in V. parahaemolyticus VBNC cells we found 509 induced genes and 309 repressed by more than 4-fold among 4820 investigated genes. Upregulation was dominant in most of non-metabolism functional categories, while five metabolism-related functional categories revealed downregulation in VBNC state. To our knowledge, this is the first study of comprehensive transcriptomic analyses of three phases of V. parahaemolyticus RIMD2210633. Although the mechanism of VBNC state is not yet clear, massive regulation of gene expression occurs in VBNC state compared with expression in other two phases, indicating VBNC cells are active.",
keywords = "Vibrio parahaemolyticus, whole genome gene expression, microarray, viable but not culturable state, VBNC, exponential phase, early stationary phase, ESCHERICHIA-COLI O157-H7, REAL-TIME PCR, NONCULTURABLE STATE, BIOFILM FORMATION, ADAPTIVE MUTAGENESIS, MESSENGER-RNA, CHOLERAE O1, IN-SITU, BACTERIA, VULNIFICUS",
author = "Lu Meng and Thomas Alter and Tommi Aho and Stephan Huehn",
year = "2015",
month = "5",
doi = "10.1093/femsec/fiv035",
language = "English",
volume = "91",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Gene expression profiles of Vibrio parahaemolyticus in viable but non-culturable state

AU - Meng, Lu

AU - Alter, Thomas

AU - Aho, Tommi

AU - Huehn, Stephan

PY - 2015/5

Y1 - 2015/5

N2 - Viable but non-culturable (VBNC) state is referred to as a dormant state of non-sporulating bacteria enhancing the survival in adverse environments. To our knowledge, only few studies have been conducted on whole genomic expression of Vibrio parahaemolyticus VBNC state. Since a degradation of nucleic acids in V. vulnificus non-culturable state has been detected, we hypothesize that gene regulation of VBNC cells is highly reduced, downregulation of gene expression is dominant and only metabolic functions crucial for survival are kept on a sustained basis. Hence, we performed the whole transcriptomic profiles of V. parahaemolyticus in three phases (exponential, early stationary phase and VBNC state). Compared with exponential and early stationary phase, in V. parahaemolyticus VBNC cells we found 509 induced genes and 309 repressed by more than 4-fold among 4820 investigated genes. Upregulation was dominant in most of non-metabolism functional categories, while five metabolism-related functional categories revealed downregulation in VBNC state. To our knowledge, this is the first study of comprehensive transcriptomic analyses of three phases of V. parahaemolyticus RIMD2210633. Although the mechanism of VBNC state is not yet clear, massive regulation of gene expression occurs in VBNC state compared with expression in other two phases, indicating VBNC cells are active.

AB - Viable but non-culturable (VBNC) state is referred to as a dormant state of non-sporulating bacteria enhancing the survival in adverse environments. To our knowledge, only few studies have been conducted on whole genomic expression of Vibrio parahaemolyticus VBNC state. Since a degradation of nucleic acids in V. vulnificus non-culturable state has been detected, we hypothesize that gene regulation of VBNC cells is highly reduced, downregulation of gene expression is dominant and only metabolic functions crucial for survival are kept on a sustained basis. Hence, we performed the whole transcriptomic profiles of V. parahaemolyticus in three phases (exponential, early stationary phase and VBNC state). Compared with exponential and early stationary phase, in V. parahaemolyticus VBNC cells we found 509 induced genes and 309 repressed by more than 4-fold among 4820 investigated genes. Upregulation was dominant in most of non-metabolism functional categories, while five metabolism-related functional categories revealed downregulation in VBNC state. To our knowledge, this is the first study of comprehensive transcriptomic analyses of three phases of V. parahaemolyticus RIMD2210633. Although the mechanism of VBNC state is not yet clear, massive regulation of gene expression occurs in VBNC state compared with expression in other two phases, indicating VBNC cells are active.

KW - Vibrio parahaemolyticus

KW - whole genome gene expression

KW - microarray

KW - viable but not culturable state

KW - VBNC

KW - exponential phase

KW - early stationary phase

KW - ESCHERICHIA-COLI O157-H7

KW - REAL-TIME PCR

KW - NONCULTURABLE STATE

KW - BIOFILM FORMATION

KW - ADAPTIVE MUTAGENESIS

KW - MESSENGER-RNA

KW - CHOLERAE O1

KW - IN-SITU

KW - BACTERIA

KW - VULNIFICUS

U2 - 10.1093/femsec/fiv035

DO - 10.1093/femsec/fiv035

M3 - Article

VL - 91

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 5

M1 - 035

ER -