Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules
Research output: Contribution to journal › Article › Scientific › peer-review
|Number of pages||5|
|Publication status||Published - Sep 2011|
|Publication type||A1 Journal article-refereed|
We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.