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Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules

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Details

Original languageEnglish
Pages (from-to)3788-3792
Number of pages5
JournalNanoscale
Volume3
Issue number9
DOIs
Publication statusPublished - Sep 2011
Publication typeA1 Journal article-refereed

Abstract

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

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