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Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium

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Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium. / Calejo, Maria Teresa; Ilmarinen, Tanja; Jongprasitkul, Hatai; Skottman, Heli; Kellomäki, Minna.

In: Journal of Biomedical Materials Research. Part A, Vol. 104, No. 7, 01.07.2016, p. 1646-1656.

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Calejo, Maria Teresa ; Ilmarinen, Tanja ; Jongprasitkul, Hatai ; Skottman, Heli ; Kellomäki, Minna. / Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium. In: Journal of Biomedical Materials Research. Part A. 2016 ; Vol. 104, No. 7. pp. 1646-1656.

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@article{4ba1a7d0a758465b90da7d37f483c59c,
title = "Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium",
abstract = "Age-related macular degeneration (AMD) is a leading cause of blindness in developed countries, characterised by the degeneration of the retinal pigment epithelium (RPE), a pigmented cell monolayer that closely interacts with the photoreceptors. RPE transplantation is thus considered a very promising therapeutic option to treat this disease. In this work, porous honeycomb-like films are for the first time investigated as scaffold materials for human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). By changing the conditions during film preparation, it was possible to produce films with homogeneous pore distribution and adequate pore size (3-5 μm), that is large enough to ensure high permeability but small enough to enable cell adherence and spreading. A brief dip-coating procedure with collagen type IV enabled the homogeneous adsorption of the protein to the walls and bottom of pores, increasing the hydrophilicity of the surface. hESC-RPE adhered and proliferated on all the collagen-coated materials, regardless of small differences in pore size. The differentiation of hESC-RPE was confirmed by the detection of specific RPE protein markers. These results suggest that the porous honeycomb films can be promising candidates for hESC-RPE tissue engineering, importantly enabling the free flow of ions and molecules across the material.",
keywords = "honeycomb films, permeability, pluripotent stem cells, retinal pigment epithelium, tissue engineering",
author = "Calejo, {Maria Teresa} and Tanja Ilmarinen and Hatai Jongprasitkul and Heli Skottman and Minna Kellom{\"a}ki",
year = "2016",
month = "7",
day = "1",
doi = "10.1002/jbm.a.35690",
language = "English",
volume = "104",
pages = "1646--1656",
journal = "Journal of Biomedical Materials Research. Part A",
issn = "1549-3296",
publisher = "Wiley",
number = "7",

}

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TY - JOUR

T1 - Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium

AU - Calejo, Maria Teresa

AU - Ilmarinen, Tanja

AU - Jongprasitkul, Hatai

AU - Skottman, Heli

AU - Kellomäki, Minna

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Age-related macular degeneration (AMD) is a leading cause of blindness in developed countries, characterised by the degeneration of the retinal pigment epithelium (RPE), a pigmented cell monolayer that closely interacts with the photoreceptors. RPE transplantation is thus considered a very promising therapeutic option to treat this disease. In this work, porous honeycomb-like films are for the first time investigated as scaffold materials for human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). By changing the conditions during film preparation, it was possible to produce films with homogeneous pore distribution and adequate pore size (3-5 μm), that is large enough to ensure high permeability but small enough to enable cell adherence and spreading. A brief dip-coating procedure with collagen type IV enabled the homogeneous adsorption of the protein to the walls and bottom of pores, increasing the hydrophilicity of the surface. hESC-RPE adhered and proliferated on all the collagen-coated materials, regardless of small differences in pore size. The differentiation of hESC-RPE was confirmed by the detection of specific RPE protein markers. These results suggest that the porous honeycomb films can be promising candidates for hESC-RPE tissue engineering, importantly enabling the free flow of ions and molecules across the material.

AB - Age-related macular degeneration (AMD) is a leading cause of blindness in developed countries, characterised by the degeneration of the retinal pigment epithelium (RPE), a pigmented cell monolayer that closely interacts with the photoreceptors. RPE transplantation is thus considered a very promising therapeutic option to treat this disease. In this work, porous honeycomb-like films are for the first time investigated as scaffold materials for human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). By changing the conditions during film preparation, it was possible to produce films with homogeneous pore distribution and adequate pore size (3-5 μm), that is large enough to ensure high permeability but small enough to enable cell adherence and spreading. A brief dip-coating procedure with collagen type IV enabled the homogeneous adsorption of the protein to the walls and bottom of pores, increasing the hydrophilicity of the surface. hESC-RPE adhered and proliferated on all the collagen-coated materials, regardless of small differences in pore size. The differentiation of hESC-RPE was confirmed by the detection of specific RPE protein markers. These results suggest that the porous honeycomb films can be promising candidates for hESC-RPE tissue engineering, importantly enabling the free flow of ions and molecules across the material.

KW - honeycomb films

KW - permeability

KW - pluripotent stem cells

KW - retinal pigment epithelium

KW - tissue engineering

U2 - 10.1002/jbm.a.35690

DO - 10.1002/jbm.a.35690

M3 - Article

VL - 104

SP - 1646

EP - 1656

JO - Journal of Biomedical Materials Research. Part A

JF - Journal of Biomedical Materials Research. Part A

SN - 1549-3296

IS - 7

ER -