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Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy

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Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy. / Zorzi, Giovanni Konat; Párraga, Jenny Evelin; Seijo, Begoña; Sánchez, Alejandro.

In: MACROMOLECULAR BIOSCIENCE, Vol. 11, No. 7, 07.07.2011, p. 905-913.

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Zorzi, Giovanni Konat ; Párraga, Jenny Evelin ; Seijo, Begoña ; Sánchez, Alejandro. / Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy. In: MACROMOLECULAR BIOSCIENCE. 2011 ; Vol. 11, No. 7. pp. 905-913.

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@article{a964420924344d4ea580d6c8d951613d,
title = "Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy",
abstract = "We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface. The physicochemical properties of the nanoparticles that impact their bioperformance, such as average size and zeta potential, can be conveniently modulated by changing the ratio of polymers and the crosslinker. These systems associate plasmid DNA and are able to protect it from DNase I degradation. We corroborate that the introduction of CS or DS in the formulation decreases the in vitro toxicity of the nanoparticles to human corneal cells without compromising the transfection efficiency. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy. New hybrid nanoparticles composed of cationized gelatin and natural polyanions are developed and characterized. The incorporation of chondroitin sulfate or dextran sulfate in cationized gelatin nanoparticles decreases their toxicity while preserving their transfection efficiency in human corneal cells. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy.",
keywords = "Drug delivery systems, Gelation, Nanoparticles, Nanotechnology",
author = "Zorzi, {Giovanni Konat} and P{\'a}rraga, {Jenny Evelin} and Bego{\~n}a Seijo and Alejandro S{\'a}nchez",
year = "2011",
month = "7",
day = "7",
doi = "10.1002/mabi.201100005",
language = "English",
volume = "11",
pages = "905--913",
journal = "MACROMOLECULAR BIOSCIENCE",
issn = "1616-5187",
publisher = "Wiley-VCH Verlag",
number = "7",

}

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TY - JOUR

T1 - Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy

AU - Zorzi, Giovanni Konat

AU - Párraga, Jenny Evelin

AU - Seijo, Begoña

AU - Sánchez, Alejandro

PY - 2011/7/7

Y1 - 2011/7/7

N2 - We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface. The physicochemical properties of the nanoparticles that impact their bioperformance, such as average size and zeta potential, can be conveniently modulated by changing the ratio of polymers and the crosslinker. These systems associate plasmid DNA and are able to protect it from DNase I degradation. We corroborate that the introduction of CS or DS in the formulation decreases the in vitro toxicity of the nanoparticles to human corneal cells without compromising the transfection efficiency. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy. New hybrid nanoparticles composed of cationized gelatin and natural polyanions are developed and characterized. The incorporation of chondroitin sulfate or dextran sulfate in cationized gelatin nanoparticles decreases their toxicity while preserving their transfection efficiency in human corneal cells. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy.

AB - We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface. The physicochemical properties of the nanoparticles that impact their bioperformance, such as average size and zeta potential, can be conveniently modulated by changing the ratio of polymers and the crosslinker. These systems associate plasmid DNA and are able to protect it from DNase I degradation. We corroborate that the introduction of CS or DS in the formulation decreases the in vitro toxicity of the nanoparticles to human corneal cells without compromising the transfection efficiency. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy. New hybrid nanoparticles composed of cationized gelatin and natural polyanions are developed and characterized. The incorporation of chondroitin sulfate or dextran sulfate in cationized gelatin nanoparticles decreases their toxicity while preserving their transfection efficiency in human corneal cells. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy.

KW - Drug delivery systems

KW - Gelation

KW - Nanoparticles

KW - Nanotechnology

UR - http://www.scopus.com/inward/record.url?scp=79959848036&partnerID=8YFLogxK

U2 - 10.1002/mabi.201100005

DO - 10.1002/mabi.201100005

M3 - Article

VL - 11

SP - 905

EP - 913

JO - MACROMOLECULAR BIOSCIENCE

JF - MACROMOLECULAR BIOSCIENCE

SN - 1616-5187

IS - 7

ER -