Tampere University of Technology

TUTCRIS Research Portal

Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy

Research output: Contribution to journalArticleScientificpeer-review

Standard

Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy. / Saari, S.; Järvinen, S.; Reponen, T.; Mensah-Attipoe, J.; Pasanen, P.; Toivonen, J.; Keskinen, Jorma.

In: Aerosol Science and Technology, Vol. 50, No. 2, 01.02.2016, p. 126-132.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

APA

Vancouver

Author

Saari, S. ; Järvinen, S. ; Reponen, T. ; Mensah-Attipoe, J. ; Pasanen, P. ; Toivonen, J. ; Keskinen, Jorma. / Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy. In: Aerosol Science and Technology. 2016 ; Vol. 50, No. 2. pp. 126-132.

Bibtex - Download

@article{3779afde0e6444a58947f76c35639842,
title = "Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy",
abstract = "Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 × 10-12 g/particle) compared to fungal spores (3-5 × 10-16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.",
author = "S. Saari and S. J{\"a}rvinen and T. Reponen and J. Mensah-Attipoe and P. Pasanen and J. Toivonen and Jorma Keskinen",
year = "2016",
month = "2",
day = "1",
doi = "10.1080/02786826.2015.1134764",
language = "English",
volume = "50",
pages = "126--132",
journal = "Aerosol Science and Technology",
issn = "0278-6826",
publisher = "Taylor & Francis",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Identification of single microbial particles using electro-dynamic balance assisted laser-induced breakdown and fluorescence spectroscopy

AU - Saari, S.

AU - Järvinen, S.

AU - Reponen, T.

AU - Mensah-Attipoe, J.

AU - Pasanen, P.

AU - Toivonen, J.

AU - Keskinen, Jorma

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 × 10-12 g/particle) compared to fungal spores (3-5 × 10-16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.

AB - Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 × 10-12 g/particle) compared to fungal spores (3-5 × 10-16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.

U2 - 10.1080/02786826.2015.1134764

DO - 10.1080/02786826.2015.1134764

M3 - Article

VL - 50

SP - 126

EP - 132

JO - Aerosol Science and Technology

JF - Aerosol Science and Technology

SN - 0278-6826

IS - 2

ER -