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Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas

Research output: Contribution to journalArticleScientificpeer-review


Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalBioresource Technology
Publication statusPublished - 1 Nov 2014
Publication typeA1 Journal article-refereed


Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561mg/Ld) and fatty-acid methyl-ester (168mg/Ld) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.


  • Chlorella sp., Coal-fired flue-gas, Fed-batch, Lipid, Mixotrophic culture