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Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer: Kinetic, metabolic and transcription analyses

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Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer : Kinetic, metabolic and transcription analyses. / Ciranna, Alessandro; Ferrari, Roberto; Santala, Ville; Karp, Matti.

In: International Journal of Hydrogen Energy, Vol. 39, No. 12, 15.04.2014, p. 6391-6401.

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Ciranna, Alessandro ; Ferrari, Roberto ; Santala, Ville ; Karp, Matti. / Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer : Kinetic, metabolic and transcription analyses. In: International Journal of Hydrogen Energy. 2014 ; Vol. 39, No. 12. pp. 6391-6401.

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@article{42f73d9544cb4cce9c81f23c4bd4753d,
title = "Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer: Kinetic, metabolic and transcription analyses",
abstract = "In this study the tolerance of the alkalithermophile Caloramator celer towards substrate (glucose) and soluble end product (acetate, formate and ethanol) inhibition was assessed employing nonlinear inhibition models. In addition, the effects of subinhibitory concentrations of end products on fermentative metabolism and regulation of 12 key genes involved in pyruvate catabolism were studied. Optimal growth and H2 production were found at 50 mM of glucose and the critical substrate concentration was observed at 290-360 mM. Two inhibition models revealed that ethanol had a higher inhibitory effect on growth rate, whereas H2 production kinetics was more sensitive towards increasing concentrations of acetate and formate. Acetate, the main soluble metabolite of the fermentation, inhibited the H2 production by increasing the ionic strength in the medium. Subinhibitory concentrations of soluble end products induced changes in the metabolite profile of C. celer, specifically exogenous acetate (80 mM) and ethanol (40 mM) slightly increased the H2 yield by 4 and 7{\%}, respectively. However, despite the observed metabolic shifts, gene regulation was minimal and not always in agreement with the measured product yields. Overall, the results suggest that further optimization of the H2 production process from C. celer should focus on methods to evolve adapted osmotolerant strains and/or remove soluble metabolites, especially acetate, from the culture. Copyright {\circledC} 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.",
keywords = "Acetate, Dark fermentation, End product inhibition, Gene expression, Kinetic model, Substrate inhibition",
author = "Alessandro Ciranna and Roberto Ferrari and Ville Santala and Matti Karp",
note = "Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-04-29<br/>Publisher name: Elsevier Ltd; International Association for Hydrogen Energy",
year = "2014",
month = "4",
day = "15",
doi = "10.1016/j.ijhydene.2014.02.047",
language = "English",
volume = "39",
pages = "6391--6401",
journal = "International Journal of Hydrogen Energy",
issn = "0360-3199",
publisher = "Elsevier",
number = "12",

}

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TY - JOUR

T1 - Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer

T2 - Kinetic, metabolic and transcription analyses

AU - Ciranna, Alessandro

AU - Ferrari, Roberto

AU - Santala, Ville

AU - Karp, Matti

N1 - Contribution: organisation=keb,FACT1=1<br/>Portfolio EDEND: 2014-04-29<br/>Publisher name: Elsevier Ltd; International Association for Hydrogen Energy

PY - 2014/4/15

Y1 - 2014/4/15

N2 - In this study the tolerance of the alkalithermophile Caloramator celer towards substrate (glucose) and soluble end product (acetate, formate and ethanol) inhibition was assessed employing nonlinear inhibition models. In addition, the effects of subinhibitory concentrations of end products on fermentative metabolism and regulation of 12 key genes involved in pyruvate catabolism were studied. Optimal growth and H2 production were found at 50 mM of glucose and the critical substrate concentration was observed at 290-360 mM. Two inhibition models revealed that ethanol had a higher inhibitory effect on growth rate, whereas H2 production kinetics was more sensitive towards increasing concentrations of acetate and formate. Acetate, the main soluble metabolite of the fermentation, inhibited the H2 production by increasing the ionic strength in the medium. Subinhibitory concentrations of soluble end products induced changes in the metabolite profile of C. celer, specifically exogenous acetate (80 mM) and ethanol (40 mM) slightly increased the H2 yield by 4 and 7%, respectively. However, despite the observed metabolic shifts, gene regulation was minimal and not always in agreement with the measured product yields. Overall, the results suggest that further optimization of the H2 production process from C. celer should focus on methods to evolve adapted osmotolerant strains and/or remove soluble metabolites, especially acetate, from the culture. Copyright © 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

AB - In this study the tolerance of the alkalithermophile Caloramator celer towards substrate (glucose) and soluble end product (acetate, formate and ethanol) inhibition was assessed employing nonlinear inhibition models. In addition, the effects of subinhibitory concentrations of end products on fermentative metabolism and regulation of 12 key genes involved in pyruvate catabolism were studied. Optimal growth and H2 production were found at 50 mM of glucose and the critical substrate concentration was observed at 290-360 mM. Two inhibition models revealed that ethanol had a higher inhibitory effect on growth rate, whereas H2 production kinetics was more sensitive towards increasing concentrations of acetate and formate. Acetate, the main soluble metabolite of the fermentation, inhibited the H2 production by increasing the ionic strength in the medium. Subinhibitory concentrations of soluble end products induced changes in the metabolite profile of C. celer, specifically exogenous acetate (80 mM) and ethanol (40 mM) slightly increased the H2 yield by 4 and 7%, respectively. However, despite the observed metabolic shifts, gene regulation was minimal and not always in agreement with the measured product yields. Overall, the results suggest that further optimization of the H2 production process from C. celer should focus on methods to evolve adapted osmotolerant strains and/or remove soluble metabolites, especially acetate, from the culture. Copyright © 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

KW - Acetate

KW - Dark fermentation

KW - End product inhibition

KW - Gene expression

KW - Kinetic model

KW - Substrate inhibition

UR - http://www.scopus.com/inward/record.url?scp=84897389272&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2014.02.047

DO - 10.1016/j.ijhydene.2014.02.047

M3 - Article

VL - 39

SP - 6391

EP - 6401

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

IS - 12

ER -