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Inoculum pretreatment differentially affects the active microbial community performing mesophilic and thermophilic dark fermentation of xylose

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Details

Original languageEnglish
Pages (from-to)9233-9245
JournalInternational Journal of Hydrogen Energy
Volume43
Issue number19
Early online date1 Jan 2018
DOIs
Publication statusPublished - 10 May 2018
Publication typeA1 Journal article-refereed

Abstract

The influence of different inoculum pretreatments (pH and temperature shocks) on mesophilic (37 °C) and thermophilic (55 °C) dark fermentative H2 production from xylose (50 mM) and, for the first time, on the composition of the active microbial community was evaluated. At 37 °C, an acidic shock (pH 3, 24 h) resulted in the highest yield of 0.8 mol H2 mol−1 xylose. The H2 and butyrate yield correlated with the relative abundance of Clostridiaceae in the mesophilic active microbial community, whereas Lactobacillaceae were the most abundant non-hydrogenic competitors according to RNA-based analysis. At 55 °C, Clostridium and Thermoanaerobacterium were linked to H2 production, but only an alkaline shock (pH 10, 24 h) repressed lactate production, resulting in the highest yield of 1.2 mol H2 mol−1 xylose. This study showed that pretreatments differentially affect the structure and productivity of the active mesophilic and thermophilic microbial community developed from an inoculum.

Keywords

  • Biohydrogen, Clostridium, Lactobacillus, MiSeq, pH shock, Temperature shock

Publication forum classification

Field of science, Statistics Finland