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Kinetics of the cellular intake of a gene expression inducer at high concentrations in the media

Research output: Other conference contributionPaper, poster or abstractScientific

Details

Original languageEnglish
Publication statusPublished - 20 Mar 2015
Publication typeNot Eligible
Event2015 Tampere Meeting on Single Cell Measurements and Analysis - Tampere, Finland
Duration: 30 Mar 201530 Mar 2015

Conference

Conference2015 Tampere Meeting on Single Cell Measurements and Analysis
CountryFinland
CityTampere
Period30/03/1530/03/15

Abstract

From in vivo single-event measurements of the transient and steady-state transcription activity of a single-copy lac-ara-1 promoter in Escherichia coli, we study the intake kinetics of its inducer (IPTG) from the media. We show that the empirical data is well-fit by a model of intake that assumes a bilayer membrane coupled to a stochastic, multi-step transcription process. Next, using this model, we show that for a wide range of extracellular inducer levels (up to 1.25mM) the intake process is diffusive-like, suggesting unsaturated membrane permeability. Finally, we infer from the model that an inducer molecule takes, on average, 31.7 minutes to travel from the periplasm to the cytoplasm, which suggests that this event is a key rate-limiting process in the response time of the cells to the appearance of inducers in the media. The methodology here proposed should be of assistance to future studies of cellular intake mechanisms at the single-cell level.

Keywords

  • single cell, intake kinetics, gene expression inducer, Escherichia coli