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Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

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Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering. / Calejo, Maria Teresa; Ilmarinen, Tanja; Vuorimaa-Laukkanen, Elina; Talvitie, Elina; Hakola, Hanna M.; Skottman, Heli; Kellomäki, Minna.

In: Acta Biomaterialia, 20.02.2017.

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Calejo, Maria Teresa ; Ilmarinen, Tanja ; Vuorimaa-Laukkanen, Elina ; Talvitie, Elina ; Hakola, Hanna M. ; Skottman, Heli ; Kellomäki, Minna. / Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering. In: Acta Biomaterialia. 2017.

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@article{d390b140c12a4f93a8a24ecf069fc877,
title = "Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering",
abstract = "Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye – the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch’s membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch’s membranes for hESC-RPE transplantation.",
keywords = "Biomaterials, Tissue Engineering, Films, Retinal pigment epithelial cell, Langmuir-Schaefer film",
author = "Calejo, {Maria Teresa} and Tanja Ilmarinen and Elina Vuorimaa-Laukkanen and Elina Talvitie and Hakola, {Hanna M.} and Heli Skottman and Minna Kellom{\"a}ki",
year = "2017",
month = "2",
day = "20",
doi = "10.1016/j.actbio.2017.02.035",
language = "English",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

AU - Calejo, Maria Teresa

AU - Ilmarinen, Tanja

AU - Vuorimaa-Laukkanen, Elina

AU - Talvitie, Elina

AU - Hakola, Hanna M.

AU - Skottman, Heli

AU - Kellomäki, Minna

PY - 2017/2/20

Y1 - 2017/2/20

N2 - Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye – the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch’s membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch’s membranes for hESC-RPE transplantation.

AB - Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye – the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch’s membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch’s membranes for hESC-RPE transplantation.

KW - Biomaterials

KW - Tissue Engineering

KW - Films

KW - Retinal pigment epithelial cell

KW - Langmuir-Schaefer film

U2 - 10.1016/j.actbio.2017.02.035

DO - 10.1016/j.actbio.2017.02.035

M3 - Article

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -