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Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains

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Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains. / Heger, Michal; Salles, Isabelle I.; Bezemer, Rick; Cloos, Martijn A.; Mordon, Serge R.; Bégu, Sylvie; Deckmyn, Hans; Beek, Johan F.

In: JOURNAL OF DERMATOLOGICAL SCIENCE, Vol. 63, No. 3, 09.2011, p. 139-147.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Heger, M, Salles, II, Bezemer, R, Cloos, MA, Mordon, SR, Bégu, S, Deckmyn, H & Beek, JF 2011, 'Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains', JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 63, no. 3, pp. 139-147. https://doi.org/10.1016/j.jdermsci.2011.04.015

APA

Heger, M., Salles, I. I., Bezemer, R., Cloos, M. A., Mordon, S. R., Bégu, S., ... Beek, J. F. (2011). Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains. JOURNAL OF DERMATOLOGICAL SCIENCE, 63(3), 139-147. https://doi.org/10.1016/j.jdermsci.2011.04.015

Vancouver

Heger M, Salles II, Bezemer R, Cloos MA, Mordon SR, Bégu S et al. Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains. JOURNAL OF DERMATOLOGICAL SCIENCE. 2011 Sep;63(3):139-147. https://doi.org/10.1016/j.jdermsci.2011.04.015

Author

Heger, Michal ; Salles, Isabelle I. ; Bezemer, Rick ; Cloos, Martijn A. ; Mordon, Serge R. ; Bégu, Sylvie ; Deckmyn, Hans ; Beek, Johan F. / Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains. In: JOURNAL OF DERMATOLOGICAL SCIENCE. 2011 ; Vol. 63, No. 3. pp. 139-147.

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@article{8a15ff175054488a877e48dc2182c1ea,
title = "Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains",
abstract = "Background: Superficial vascular anomalies such as port wine stains are commonly treated by selective photothermolysis (SP). The endovascular laser-tissue interactions underlying SP are governed by a photothermal response (thermocoagulation of blood) and a hemodynamic response (thrombosis). Currently it is not known whether the hemodynamic response encompasses both primary and secondary hemostasis, which platelet receptors are involved, and what the SP-induced thrombosis kinetics are in low-flow venules. Objectives: To (1) define the role and kinetics of primary and secondary hemostasis in laser-induced thrombus formation and (2) determine which key platelet surface receptors are involved in the hemodynamic response. Methods: 532-nm laser-irradiated hamster dorsal skin fold venules were studied by intravital fluorescence microscopy following fluorescent labeling of platelets with 5(6)-carboxyfluorescein. Heparin and fluorescently labeled anti-glycoprotein Ib-α (GPIbα) and anti-P-selectin antibodies were administered to investigate the role of coagulation and platelet receptors, respectively. Lesional sizes were quantified by software. Results: Laser irradiation consistently produced sub-occlusive thermal coagula. Thrombosis was triggered in all irradiated venules in a thermal coagulum-independent manner and peaked at 6.25. min post-irradiation. Heparin decreased the maximum thrombus size and caused thrombosis to reach a maximum at 1.25. min. Immunoblocking of GPIbα abated the extent of thrombosis, whereas immunoblocking of P-selectin had no effect. Conclusions: The hemodynamic response ensues the photothermal response in a thermal coagulum-independent manner and involves primary and secondary hemostasis. Primary hemostasis is mediated by constitutively expressed GPIbα but not by activation-dependent P-selectin.",
keywords = "Glycoprotein Ib-alpha, Hamster dorsal skinfold model, P-selectin, Port wine stains, Site-specific pharmaco-laser therapy, Thermosensitive liposomes",
author = "Michal Heger and Salles, {Isabelle I.} and Rick Bezemer and Cloos, {Martijn A.} and Mordon, {Serge R.} and Sylvie B{\'e}gu and Hans Deckmyn and Beek, {Johan F.}",
year = "2011",
month = "9",
doi = "10.1016/j.jdermsci.2011.04.015",
language = "English",
volume = "63",
pages = "139--147",
journal = "JOURNAL OF DERMATOLOGICAL SCIENCE",
issn = "0923-1811",
publisher = "Elsevier",
number = "3",

}

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TY - JOUR

T1 - Laser-induced primary and secondary hemostasis dynamics and mechanisms in relation to selective photothermolysis of port wine stains

AU - Heger, Michal

AU - Salles, Isabelle I.

AU - Bezemer, Rick

AU - Cloos, Martijn A.

AU - Mordon, Serge R.

AU - Bégu, Sylvie

AU - Deckmyn, Hans

AU - Beek, Johan F.

PY - 2011/9

Y1 - 2011/9

N2 - Background: Superficial vascular anomalies such as port wine stains are commonly treated by selective photothermolysis (SP). The endovascular laser-tissue interactions underlying SP are governed by a photothermal response (thermocoagulation of blood) and a hemodynamic response (thrombosis). Currently it is not known whether the hemodynamic response encompasses both primary and secondary hemostasis, which platelet receptors are involved, and what the SP-induced thrombosis kinetics are in low-flow venules. Objectives: To (1) define the role and kinetics of primary and secondary hemostasis in laser-induced thrombus formation and (2) determine which key platelet surface receptors are involved in the hemodynamic response. Methods: 532-nm laser-irradiated hamster dorsal skin fold venules were studied by intravital fluorescence microscopy following fluorescent labeling of platelets with 5(6)-carboxyfluorescein. Heparin and fluorescently labeled anti-glycoprotein Ib-α (GPIbα) and anti-P-selectin antibodies were administered to investigate the role of coagulation and platelet receptors, respectively. Lesional sizes were quantified by software. Results: Laser irradiation consistently produced sub-occlusive thermal coagula. Thrombosis was triggered in all irradiated venules in a thermal coagulum-independent manner and peaked at 6.25. min post-irradiation. Heparin decreased the maximum thrombus size and caused thrombosis to reach a maximum at 1.25. min. Immunoblocking of GPIbα abated the extent of thrombosis, whereas immunoblocking of P-selectin had no effect. Conclusions: The hemodynamic response ensues the photothermal response in a thermal coagulum-independent manner and involves primary and secondary hemostasis. Primary hemostasis is mediated by constitutively expressed GPIbα but not by activation-dependent P-selectin.

AB - Background: Superficial vascular anomalies such as port wine stains are commonly treated by selective photothermolysis (SP). The endovascular laser-tissue interactions underlying SP are governed by a photothermal response (thermocoagulation of blood) and a hemodynamic response (thrombosis). Currently it is not known whether the hemodynamic response encompasses both primary and secondary hemostasis, which platelet receptors are involved, and what the SP-induced thrombosis kinetics are in low-flow venules. Objectives: To (1) define the role and kinetics of primary and secondary hemostasis in laser-induced thrombus formation and (2) determine which key platelet surface receptors are involved in the hemodynamic response. Methods: 532-nm laser-irradiated hamster dorsal skin fold venules were studied by intravital fluorescence microscopy following fluorescent labeling of platelets with 5(6)-carboxyfluorescein. Heparin and fluorescently labeled anti-glycoprotein Ib-α (GPIbα) and anti-P-selectin antibodies were administered to investigate the role of coagulation and platelet receptors, respectively. Lesional sizes were quantified by software. Results: Laser irradiation consistently produced sub-occlusive thermal coagula. Thrombosis was triggered in all irradiated venules in a thermal coagulum-independent manner and peaked at 6.25. min post-irradiation. Heparin decreased the maximum thrombus size and caused thrombosis to reach a maximum at 1.25. min. Immunoblocking of GPIbα abated the extent of thrombosis, whereas immunoblocking of P-selectin had no effect. Conclusions: The hemodynamic response ensues the photothermal response in a thermal coagulum-independent manner and involves primary and secondary hemostasis. Primary hemostasis is mediated by constitutively expressed GPIbα but not by activation-dependent P-selectin.

KW - Glycoprotein Ib-alpha

KW - Hamster dorsal skinfold model

KW - P-selectin

KW - Port wine stains

KW - Site-specific pharmaco-laser therapy

KW - Thermosensitive liposomes

UR - http://www.scopus.com/inward/record.url?scp=79961025227&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2011.04.015

DO - 10.1016/j.jdermsci.2011.04.015

M3 - Article

VL - 63

SP - 139

EP - 147

JO - JOURNAL OF DERMATOLOGICAL SCIENCE

JF - JOURNAL OF DERMATOLOGICAL SCIENCE

SN - 0923-1811

IS - 3

ER -