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Lipid production by eukaryotic microorganisms isolated from palm oil mill effluent

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Details

Original languageEnglish
Pages (from-to)48-54
Number of pages7
JournalBiochemical Engineering Journal
Volume99
DOIs
Publication statusPublished - 5 Jul 2015
Publication typeA1 Journal article-refereed

Abstract

Microbial oil production combined with wastewater management is one option for a more sustainable future. Micrographs of microbial cultures enriched from palm oil mill effluent (POME) showed lipid inclusion in the eukaryotic cells, indicating the cells can accumulate lipids. However, enriching the culture did not increase the total lipids. Therefore, eukaryotic microorganisms were isolated from POME to investigate whether these microorganisms are potential lipid producers. Four strains were isolated, and their lipid synthesis capabilities were compared with known oleaginous yeasts in a synthetic oil-free medium. Two strains (identified as Galactomyces geotrichum and Graphium penicillioides) had the potential to accumulate lipid accumulation based on the increase in triacylglycerol content. G. penicillioides was the most promising strain for lipid production as this strain accumulated more lipids than the well-known oleaginous yeast Cryptococcus curvatus (29.1 ± 3.0. wt% vs. 20.2 ± 2.9. wt%). To our knowledge, oil synthesis and accumulation by G. penicillioides have not previously been reported.

Keywords

  • Filamentous fungi, Lipid accumulation, Microbial growth, Palm oil mill effluent, Physiology, Yeast

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