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Long-chain GM1 gangliosides alter transmembrane domain registration through interdigitation

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Details

Original languageEnglish
Pages (from-to)870-878
Number of pages9
JournalBiochimica et Biophysica Acta: Biomembranes
Volume1859
Issue number5
DOIs
Publication statusPublished - 1 May 2017
Publication typeA1 Journal article-refereed

Abstract

Extracellular and cytosolic leaflets in cellular membranes are distinctly different in lipid composition, yet they contribute together to signaling across the membranes. Here we consider a mechanism based on long-chain gangliosides for coupling the extracellular and cytosolic membrane leaflets together. Based on atomistic molecular dynamics simulations, we find that long-chain GM1 in the extracellular leaflet exhibits a strong tendency to protrude into the opposing bilayer leaflet. This interdigitation modulates the order in the cytosolic monolayer and thereby strengthens the interaction and coupling across a membrane. Coarse-grained simulations probing longer time scales in large membrane systems indicate that GM1 in the extracellular leaflet modulates the phase behavior in the cytosolic monolayer. While short-chain GM1 maintains phase-symmetric bilayers with a strong membrane registration effect, the situation is altered with long-chain GM1. Here, the significant interdigitation induced by long-chain GM1 modulates the behavior in the cytosolic GM1-free leaflet, weakening and slowing down the membrane registration process. The observed physical interaction mechanism provides a possible means to mediate or foster transmembrane communication associated with signal transduction.

ASJC Scopus subject areas

Keywords

  • cholesterol, computer simulations, Glycosphingolipid, membrane domain, membrane registry, molecular dynamics

Publication forum classification

Field of science, Statistics Finland

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