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LTCC-Based Multi-Electrode Arrays for 3D in Vitro Cell Cultures

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Details

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalJournal of Ceramic Science and Technology
Volume6
Issue number4
DOIs
Publication statusPublished - Dec 2015
Publication typeA1 Journal article-refereed

Abstract

Current technologies to monitor neuronal cultures in vitro are based on 2-dimensional (2D) multi-electrode arrays and cell cultures. The complexity of actual high-level neurobiological systems requires 3-dimensional (3D) cultures and 3D electrode arrays to improve our understanding of such systems. The realization calls for smart multilayer and packaging technology. Our approach uses low-temperature cofired ceramics (LTCC) for the design of a 3-dimensional multi-electrode array (3D MEA). An LTCC multilayer board with gold electrodes forms the basis of the system. The layout of the 3D MEA is designed to fit into widely used measurement adapters for 2D signal recordings, enabling data processing identical to that of established chips. Design and manufacturing of the new 3D device as a basic tool for the investigation of 3D cell cultures are described. Features of thick-film gold electrodes are characterized by means of microscopic and spectroscopic tools complemented with complex impedance measurements. Possible biological applications for in vitro electrophysiological measurements were evaluated based on cell cultures of primary neurons, seeded directly to the chip surface. It was shown that activity can be measured over six months.

Keywords

  • Low-temperature cofired ceramics (LTCC), three-dimensional hybrid multi-electrode array (3D MEA), in vitro cell culture, thick-film gold electrode, primary neuron culture, GROWTH

Publication forum classification

Field of science, Statistics Finland