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Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

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Details

Original languageEnglish
Article number4723
Pages (from-to)78-80
Number of pages3
JournalJournal of Microbiological Methods
Volume118
DOIs
Publication statusPublished - 2015
Publication typeA1 Journal article-refereed

Abstract

A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

Keywords

  • Antioxidative activity, Bacterial biosensor, Bioscreening, Microplate technique

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